Cloned (Comment) | Organism |
---|---|
- |
Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
to 2.2 A resolution, by molecular replacement, and superimposition of the structures bound either to the S-adenosyl-L-methionine analog sinefungin or to 7-oxo-8-aminopelargonic acid. Comparison to structure of the Bacillus subtilis enzyme, EC 2.5.1.105 | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
S-adenosyl-L-methionine | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.7 | - |
sinefungin | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WQ80 | - |
- |
Mycobacterium tuberculosis CDC 1551 | P9WQ80 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 8-amino-7-oxononanoate | - |
Mycobacterium tuberculosis | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? | |
S-adenosyl-L-methionine + 8-amino-7-oxononanoate | - |
Mycobacterium tuberculosis CDC 1551 | S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate | - |
? | |
S-adenosyl-L-methionine + sinefungin | - |
Mycobacterium tuberculosis | S-adenosyl-4-methylthio-2-oxobutanoate + ? | - |
? | |
S-adenosyl-L-methionine + sinefungin | - |
Mycobacterium tuberculosis CDC 1551 | S-adenosyl-4-methylthio-2-oxobutanoate + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
sinefungin | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.012 | - |
S-adenosyl-L-methionine | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
sinefungin | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis | |
0.03 | - |
S-adenosyl-L-methionine | pH 7.5, temperature not specified in the publication | Mycobacterium tuberculosis |