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Literature summary for 2.6.1.63 extracted from

  • Harada, I.; Noguchi, T.; Kido, R.
    Purification and characterization of aromatic-amino-acid-glyoxylate aminotransferase from monkey and rat liver (1978), Hoppe-Seyler's Z. Physiol. Chem., 359, 481-488.
    View publication on PubMed
Search for more literature for 2.6.1.63

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
 Macaca fuscata
hydroxylamine
-
 Rattus norvegicus
Isonicotinic hydrazide
-
 Macaca fuscata
Isonicotinic hydrazide
-
 Rattus norvegicus
KCN
-
 Macaca fuscata
KCN
-
 Rattus norvegicus
Semicarbazide
-
 Macaca fuscata
Semicarbazide
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 glyoxylate
-
 Macaca fuscata
0.5
-
 glyoxylate
-
 Rattus norvegicus
1.2
-
 L-kynurenine
-
 Rattus norvegicus
1.5
-
 L-kynurenine
-
 Macaca fuscata

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-
mitochondrion
-
 Macaca fuscata 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Rattus norvegicus
40000
-
 2 * 40000, SDS-PAGE Macaca fuscata
80000
-
 gel filtration Rattus norvegicus
80000
-
 gel filtration Macaca fuscata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-kynurenine + glyoxylate Rattus norvegicus glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate Macaca fuscata glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate Rattus norvegicus Donryu glucagon inducible enzyme 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?

Organism

Organism UniProt Comment Textmining
Macaca fuscata
-
 Blyth monkey
-
Rattus norvegicus
-
 Donryu strain
-
Rattus norvegicus Donryu
-
 Donryu strain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Macaca fuscata

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Macaca fuscata
-

Storage Stability

Storage Stability Organism
-20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 4 weeks Macaca fuscata
-20°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, at least 7 weeks Rattus norvegicus
0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal 5’-phosphate, 5 mM 2-mercaptoethanol, 20% glycerol, 2 weeks Macaca fuscata
0-4°C, in 50 mM potassium phosphate buffer, pH 7.5, 0.2 mM pyridoxal phosphate, 5 mM mercaptoethanol, 20% glycerol, 3 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxytryptophan + glyoxylate
-
 Rattus norvegicus 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
 ?
5-hydroxytryptophan + glyoxylate
-
 Macaca fuscata 3-(5-hydroxyindole)-2-oxopropanoate + glycine
-
 ?
histidine + glyoxylate
-
 Rattus norvegicus 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ?
histidine + glyoxylate
-
 Macaca fuscata 3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
-
 ?
L-kynurenine + 2-oxoglutarate
-
 Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
-
 ?
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
 Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
 ?
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
 Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
 ?
L-kynurenine + 4-methylsulfanyl-2-oxobutanoate
-
 Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-methionine
-
 ?
L-kynurenine + glyoxylate
-
 Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate
-
 Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate glucagon inducible enzyme Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate glucagon inducible enzyme Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate
-
 Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + glyoxylate glucagon inducible enzyme Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + glycine
-
 ?
L-kynurenine + hydroxypyruvate
-
 Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
 ?
L-kynurenine + hydroxypyruvate
-
 Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
 ?
L-kynurenine + hydroxypyruvate
-
 Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-serine
-
 ?
L-kynurenine + pyruvate
-
 Rattus norvegicus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 ?
L-kynurenine + pyruvate
-
 Macaca fuscata 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 ?
L-kynurenine + pyruvate
-
 Rattus norvegicus Donryu 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 ?
phenylalanine + glyoxylate
-
 Rattus norvegicus phenylpyruvate + glycine
-
 ?
phenylalanine + glyoxylate
-
 Macaca fuscata phenylpyruvate + glycine
-
 ?
tryptophan + glyoxylate
-
 Rattus norvegicus 3-indole-2-oxopropanoate + glycine
-
 ?
tryptophan + glyoxylate
-
 Macaca fuscata 3-indole-2-oxopropanoate + glycine
-
 ?
tyrosine + glyoxylate
-
 Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
 ?
tyrosine + glyoxylate
-
 Macaca fuscata 3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE Rattus norvegicus
dimer 2 * 40000, SDS-PAGE Macaca fuscata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus
37
-
 assay at Macaca fuscata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 t1/2: 25 min Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate requirement, tightly bound Rattus norvegicus
pyridoxal 5'-phosphate requirement, tightly bound Macaca fuscata