Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Rattus norvegicus |
General Stability | Organism |
---|---|
dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates | Rattus norvegicus |
stable in phosphate buffer supplemented with pyridoxal phosphate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Aminoadipate | - |
Rattus norvegicus | |
2-oxoadipate | - |
Rattus norvegicus | |
2-oxoglutarate | substrate inhibition | Rattus norvegicus | |
adipate | - |
Rattus norvegicus | |
Co2+ | - |
Rattus norvegicus | |
Dicarboxylic acids | C-2 to C-10 | Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
hydroxylamine | complete inhibition | Rattus norvegicus | |
KCN | complete inhibition | Rattus norvegicus | |
L-kynurenine | substrate inhibition above 6 mM | Rattus norvegicus | |
additional information | not inhibited by L-3-hydroxykynurenine at higher concentrations | Rattus norvegicus | |
Ni2+ | - |
Rattus norvegicus | |
phosphate | - |
Rattus norvegicus | |
Sn2+ | - |
Rattus norvegicus | |
sulfate | - |
Rattus norvegicus | |
Sulfate or phosphate esters of steroids | - |
Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
pyridoxal 5'-phosphate | pH 6.5, 37°C | Rattus norvegicus | |
1 | - |
2-oxoglutarate | pH 6.5, 37°C | Rattus norvegicus | |
1.4 | - |
L-kynurenine | pH 6.5, 37°C | Rattus norvegicus | |
2 | - |
L-3-hydroxykynurenine | pH 6.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol | Rattus norvegicus |
45500 | - |
2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol | Rattus norvegicus |
85000 | - |
gel filtration, sucrose density gradient centrifugation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + 2-oxoglutarate | Rattus norvegicus | inducible enzyme of tryptophan catabolism | kynurenic acid + L-glutamate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
adult albino | - |
Purification (Comment) | Organism |
---|---|
acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate | transamination of the amino acid to a transient keto acid intermediate which spontaneously undergoes ring closure | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.65 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-diiodo-L-tyrosine + 2-oxoglutarate | slightly better than L-kynurenine | Rattus norvegicus | 3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? | |
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate | at 39.7% of the activity with 2-aminohexane-1,6-dioate | Rattus norvegicus | 2-oxoheptane-1,7-dioate + L-glutamate | - |
? | |
L-3-hydroxykynurenine + 2-oxoglutarate | no activity with D-isomer | Rattus norvegicus | xanthurenic acid + L-glutamate | - |
? | |
L-kynurenine + 2-oxoglutarate | - |
Rattus norvegicus | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + 2-oxoglutarate | inducible enzyme of tryptophan catabolism | Rattus norvegicus | kynurenic acid + L-glutamate + H2O | - |
? | |
L-kynurenine + 2-oxohexanedioate | - |
Rattus norvegicus | kynurenic acid + 2-aminohexanedioate + H2O | - |
? | |
L-kynurenine + pyruvate | less effective than 2-oxoglutarate | Rattus norvegicus | kynurenic acid + L-alanine + H2O | - |
? | |
L-methionine + 2-oxoglutarate | 12% the rate of L-kynurenine | Rattus norvegicus | 4-methylsulfanyl-2-oxobutanoate + glutamate | - |
? | |
L-norleucine + 2-oxoglutarate | at about 30% the rate of L-kynurenine | Rattus norvegicus | 2-oxohexanoate + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Rattus norvegicus | phenylpyruvate + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | 16% the rate of L-kynurenine | Rattus norvegicus | 3-indole-2-oxopropanoate + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Rattus norvegicus | 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol | Rattus norvegicus |
dimer | 2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
and above, 5 min, decreasing activity | Rattus norvegicus |
70 | - |
5 min, complete inactivation | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.56 | Rattus norvegicus |
6.5 | - |
with L-kynurenine as substrate | Rattus norvegicus |
7.5 | - |
with L-alpha-aminoadipate as substrate | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
and below, inactivation even in the presence of pyridoxal phosphate | Rattus norvegicus |
6.5 | 7.5 | most stable | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km-value: 0.00085 mM | Rattus norvegicus |