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Literature summary for 2.6.1.7 extracted from

  • Tobes, M.C.
    Kynurenine-oxoglutarate aminotransferase from rat kidney (1987), Methods Enzymol., 142, 217-224.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Rattus norvegicus

General Stability

General Stability Organism
dialysis of the apoenzyme against Tris-HCl or imidazole-HCl buffer inactivates Rattus norvegicus
stable in phosphate buffer supplemented with pyridoxal phosphate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-Aminoadipate
-
Rattus norvegicus
2-oxoadipate
-
Rattus norvegicus
2-oxoglutarate substrate inhibition Rattus norvegicus
adipate
-
Rattus norvegicus
Co2+
-
Rattus norvegicus
Dicarboxylic acids C-2 to C-10 Rattus norvegicus
Hg2+
-
Rattus norvegicus
hydroxylamine complete inhibition Rattus norvegicus
KCN complete inhibition Rattus norvegicus
L-kynurenine substrate inhibition above 6 mM Rattus norvegicus
additional information not inhibited by L-3-hydroxykynurenine at higher concentrations Rattus norvegicus
Ni2+
-
Rattus norvegicus
phosphate
-
Rattus norvegicus
Sn2+
-
Rattus norvegicus
sulfate
-
Rattus norvegicus
Sulfate or phosphate esters of steroids
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00085
-
pyridoxal 5'-phosphate pH 6.5, 37°C Rattus norvegicus
1
-
2-oxoglutarate pH 6.5, 37°C Rattus norvegicus
1.4
-
L-kynurenine pH 6.5, 37°C Rattus norvegicus
2
-
L-3-hydroxykynurenine pH 6.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-
soluble
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus
45500
-
2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol Rattus norvegicus
85000
-
gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-kynurenine + 2-oxoglutarate Rattus norvegicus inducible enzyme of tryptophan catabolism kynurenic acid + L-glutamate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
adult albino
-

Purification (Commentary)

Purification (Comment) Organism
acid precipitation, ammonium sulfate, DEAE-cellulose, hydroxylapatite Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate transamination of the amino acid to a transient keto acid intermediate which spontaneously undergoes ring closure Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.65
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,5-diiodo-L-tyrosine + 2-oxoglutarate slightly better than L-kynurenine Rattus norvegicus 3-(2,5-diiodo-4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
?
L-2-aminoheptane-1,7-dioate + 2-oxoglutarate at 39.7% of the activity with 2-aminohexane-1,6-dioate Rattus norvegicus 2-oxoheptane-1,7-dioate + L-glutamate
-
?
L-3-hydroxykynurenine + 2-oxoglutarate no activity with D-isomer Rattus norvegicus xanthurenic acid + L-glutamate
-
?
L-kynurenine + 2-oxoglutarate
-
Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
?
L-kynurenine + 2-oxoglutarate inducible enzyme of tryptophan catabolism Rattus norvegicus kynurenic acid + L-glutamate + H2O
-
?
L-kynurenine + 2-oxohexanedioate
-
Rattus norvegicus kynurenic acid + 2-aminohexanedioate + H2O
-
?
L-kynurenine + pyruvate less effective than 2-oxoglutarate Rattus norvegicus kynurenic acid + L-alanine + H2O
-
?
L-methionine + 2-oxoglutarate 12% the rate of L-kynurenine Rattus norvegicus 4-methylsulfanyl-2-oxobutanoate + glutamate
-
?
L-norleucine + 2-oxoglutarate at about 30% the rate of L-kynurenine Rattus norvegicus 2-oxohexanoate + L-glutamate
-
?
L-phenylalanine + 2-oxoglutarate
-
Rattus norvegicus phenylpyruvate + L-glutamate
-
?
L-tryptophan + 2-oxoglutarate 16% the rate of L-kynurenine Rattus norvegicus 3-indole-2-oxopropanoate + L-glutamate
-
?
L-tyrosine + 2-oxoglutarate
-
Rattus norvegicus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 45500, SDS-PAGE in the presence of 2-mercaptoethanol Rattus norvegicus
dimer 2 * 45000, SDS-PAGE in the absence of 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
and above, 5 min, decreasing activity Rattus norvegicus
70
-
5 min, complete inactivation Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 6.56 Rattus norvegicus
6.5
-
with L-kynurenine as substrate Rattus norvegicus
7.5
-
with L-alpha-aminoadipate as substrate Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
and below, inactivation even in the presence of pyridoxal phosphate Rattus norvegicus
6.5 7.5 most stable Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Km-value: 0.00085 mM Rattus norvegicus