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Literature summary for 2.6.1.7 extracted from

  • Han, Q.; Robinson, H.; Cai, T.; Tagle, D.A.; Li, J.
    Biochemical and structural properties of mouse kynurenine aminotransferase III (2009), Mol. Cell. Biol., 29, 784-793.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with L-kynurenine and L-glutamine, hanging drop vapor diffusion method, using 21% (w/v) polyethylene glycol 400, 10% glycerol, 150 mM CaCl2, and 100 mM HEPES, pH 7.5 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxykynurenine
-
 Mus musculus
L-cysteine
-
 Mus musculus
L-glutamine
-
 Mus musculus
L-histidine
-
 Mus musculus
L-leucine
-
 Mus musculus
L-methionine
-
 Mus musculus
additional information indol-3ylpyruvate shows no noticeable inhibition of KAT III Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 glyoxylate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.4
-
 alpha-oxo-gamma-methiol-butyric acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.5
-
 indo-3-pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.5
-
 2-oxocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.6
-
 phenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.7
-
 L-cysteine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.7
-
 L-glutamine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.7
-
 L-histidine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.9
-
 L-methionine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1
-
 2-oxobutyrate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1
-
 hydroxyphenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.1
-
 L-phenylalanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.2
-
 2-oxovalerate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.4
-
 L-asparagine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.5
-
 L-kynurenine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.4
-
 Mercaptopyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.7
-
 L-tyrosine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
3
-
 L-serine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
4.9
-
 oxaloacetate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
5.3
-
 2-oxoisocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
6.2
-
 L-alanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
7.1
-
 L-tryptophan in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
8.1
-
 2-oxoglutarate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
10.6
-
 pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q71RI9
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography, hydroxyapatite column chromatography, Mono-Q column chromatography, and gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobutyrate + glyoxylate
-
 Mus musculus ?
-
 ?
3-hydroxykynurenine + glyoxylate
-
 Mus musculus ?
-
 ?
L-alanine + glyoxylate
-
 Mus musculus ?
-
 ?
L-asparagine + glyoxylate KAT II has high transamination activity toward L-asparagine Mus musculus ?
-
 ?
L-cysteine + glyoxylate
-
 Mus musculus ?
-
 ?
L-glutamine + glyoxylate
-
 Mus musculus ?
-
 ?
L-histidine + glyoxylate
-
 Mus musculus ?
-
 ?
L-kynurenine + 2-oxobutyrate
-
 Mus musculus kynurenic acid + 2-aminobutanoate + H2O
-
 ?
L-kynurenine + 2-oxocaproic acid
-
 Mus musculus ?
-
 ?
L-kynurenine + 2-oxoglutarate poor cosubstrate for KAT III Mus musculus ?
-
 ?
L-kynurenine + 2-oxoisocaproic acid
-
 Mus musculus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-leucine
-
 ?
L-kynurenine + 2-oxomethylthiobutyric acid
-
 Mus musculus ?
-
 ?
L-kynurenine + 2-oxovalerate
-
 Mus musculus ?
-
 ?
L-kynurenine + glyoxylate
-
 Mus musculus ?
-
 ?
L-kynurenine + indol-3-ylpyruvate
-
 Mus musculus ?
-
 ?
L-kynurenine + mercaptopyruvate
-
 Mus musculus ?
-
 ?
L-kynurenine + oxaloacetate
-
 Mus musculus ?
-
 ?
L-kynurenine + p-hydroxyphenylpyruvate
-
 Mus musculus ?
-
 ?
L-kynurenine + phenylpyruvate
-
 Mus musculus ?
-
 ?
L-kynurenine + phenylpyruvate poor cosubstrate for KAT III Mus musculus ?
-
 ?
L-kynurenine + pyruvate poor cosubstrate for KAT III Mus musculus ?
-
 ?
L-lysine + glyoxylate
-
 Mus musculus ?
-
 ?
L-methionine + glyoxylate
-
 Mus musculus ?
-
 ?
L-phenylalanine + glyoxylate
-
 Mus musculus ?
-
 ?
L-serine + glyoxylate
-
 Mus musculus ?
-
 ?
L-tryptophan + glyoxylate
-
 Mus musculus ?
-
 ?
L-tyrosine + glyoxylate
-
 Mus musculus ?
-
 ?
additional information KAT III does not use L-valine, L-proline, L-threonine, L-leucine, L-isoleucine, L-glutamate, L-aspartate, L-arginine, and aminoadipate as substrates Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
KAT III
-
 Mus musculus
kynurenine aminotransferase III
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60
-
 Mus musculus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 2-oxoglutarate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.74
-
 2-oxoisocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.02
-
 alpha-oxo-gamma-methiol-butyric acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.03
-
 L-tyrosine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.03
-
 indo-3-pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.3
-
 L-cysteine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.48
-
 hydroxyphenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.53
-
 L-alanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.87
-
 pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2
-
 L-histidine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.17
-
 L-serine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.27
-
 L-glutamine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.3
-
 L-kynurenine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.43
-
 L-methionine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.53
-
 phenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.59
-
 2-oxocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.7
-
 L-phenylalanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.7
-
 2-oxovalerate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.71
-
 glyoxylate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.93
-
 L-asparagine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.93
-
 2-oxobutyrate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
3.28
-
 Mercaptopyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
3.57
-
 L-tryptophan in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
3.68
-
 oxaloacetate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
8 11
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.045
-
 2-oxoglutarate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.14
-
 2-oxoisocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.18
-
 pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.25
-
 L-alanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.38
-
 L-tyrosine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.5
-
 L-tryptophan in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.72
-
 L-serine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
0.75
-
 oxaloacetate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.37
-
 Mercaptopyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.48
-
 hydroxyphenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.53
-
 L-kynurenine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
1.86
-
 L-cysteine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.07
-
 indo-3-pyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.095
-
 L-asparagine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.25
-
 2-oxovalerate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.45
-
 L-phenylalanine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.54
-
 alpha-oxo-gamma-methiol-butyric acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.7
-
 L-methionine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.86
-
 L-histidine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
2.93
-
 2-oxobutyrate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
3.24
-
 L-glutamine in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
4.22
-
 phenylpyruvate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
5.18
-
 2-oxocaproic acid in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus
6.78
-
 glyoxylate in 100 mM boric acid buffer, pH 9.0, at 45°C Mus musculus