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Literature summary for 2.6.1.78 extracted from

  • De-Eknamkul, W.; Ellis, B.E.
    Purification and characterization of prephenate aminotransferase from Anchusa officinalis cell cultures (1988), Arch. Biochem. Biophys., 267, 87-94.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxyphenyllactic acid metabolite of rosmarinic acid Anchusa officinalis
additional information enzyme shows substrate inhibition, but no feedback inhibition by L-phenylalanine and L-tyrosine Anchusa officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
L-aspartate pH 9.0, 40°C Anchusa officinalis
0.08
-
prephenate pH 9.0, 40°C Anchusa officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis
57000
-
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis
220000
-
gel filtration Anchusa officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
prephenate + L-aspartate Anchusa officinalis transamination L-arogenate + oxaloacetate
-
?

Organism

Organism UniProt Comment Textmining
Anchusa officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 41fold to homogeneity by anion exchange chromatography, chromatofocusing using the major active fraction with an isoelectric point of 5.4, and gel filtration Anchusa officinalis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture rosmarinic acid-producing cells Anchusa officinalis
-
additional information activity during cell cycle Anchusa officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.34
-
purified enzyme Anchusa officinalis

Storage Stability

Storage Stability Organism
-70°C, purified enzyme, 50 mM Tris-HCl, pH 8.2, 5 mM 2-mercaptoethanol, 150 mM sucrose, 1 mM EDTA, stable for at least 5 months Anchusa officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-aspartate
-
Anchusa officinalis L-glutamate + oxaloacetate
-
?
4-hydroxyphenylpyruvate + L-aspartate
-
Anchusa officinalis L-tyrosine + oxaloacetate
-
?
glyoxylate + L-aspartate
-
Anchusa officinalis glycine + oxaloacetate
-
?
indolepyruvate + L-aspartate
-
Anchusa officinalis L-tryptophan + oxaloacetate
-
?
additional information substrate specificity, highest activity with 2-oxoglutarate and L-aspartate as substrates, low activity with indolepyruvate and glyoxylate Anchusa officinalis ?
-
?
phenylpyruvate + L-aspartate
-
Anchusa officinalis L-phenylalanine + oxaloacetate
-
?
prephenate + L-aspartate transamination Anchusa officinalis L-arogenate + oxaloacetate
-
?
prephenate + L-glutamate transamination, L-glutamate is about 3fold less effective than L-aspartate Anchusa officinalis L-arogenate + 2-oxoglutarate
-
?
pyruvate + L-aspartate
-
Anchusa officinalis L-alanine + oxaloacetate
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis

Synonyms

Synonyms Comment Organism
Pat
-
Anchusa officinalis
prephenate aminotransferase
-
Anchusa officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
Anchusa officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Anchusa officinalis

pI Value

Organism Comment pI Value Maximum pI Value
Anchusa officinalis minor fraction, chromatofocussing
-
5
Anchusa officinalis major fraction, chromatofocussing
-
5.4