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Literature summary for 2.6.1.79 extracted from
- Novel features of prephenate aminotransferase from cell cultures of Nicotiana silvestris (1985), Arch. Biochem. Biophys., 238, 237-246.
General Stability
| General Stability | Organism |
|---|---|
| pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay | Nicotiana sylvestris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| prephenate | substrate inhibition above 1 mM, antagonism by L-glutamate | Nicotiana sylvestris |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prephenate + L-glutamate | Nicotiana sylvestris | important step in biosynthesis of L-tyrosine and L-phenylalanine | L-arogenate + 2-oxoglutarate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana sylvestris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially by ion exchange chromatography | Nicotiana sylvestris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | stable cell line ANS-1 | Nicotiana sylvestris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Nicotiana sylvestris |
| 0.042 | - |
crude enzyme extract, substrates prephenate and L-glutamate | Nicotiana sylvestris |
| 0.073 | - |
partially purified enzyme, substrate pyruvate and L-glutamate | Nicotiana sylvestris |
| 0.077 | - |
partially purified enzyme, substrate prephenate and L-aspartate | Nicotiana sylvestris |
| 0.118 | - |
partially purified enzyme, substrate oxaloacetate and L-aspartate | Nicotiana sylvestris |
| 0.125 | - |
partially purified enzyme, substrate oxaloacetate and L-glutamate | Nicotiana sylvestris |
| 0.156 | - |
partially purified enzyme, substrate 2-oxoglutarate and L-aspartate | Nicotiana sylvestris |
| 0.157 | - |
partially purified enzyme, substrate prephenate and L-glutamate | Nicotiana sylvestris |
| 0.251 | - |
partially purified enzyme, substrate 2-oxoglutarate and L-glutamate | Nicotiana sylvestris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| in presence of pyridoxal 5'-phosphate, EDTA, glycerol, stable for at least one month | Nicotiana sylvestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + L-aspartate | - |
Nicotiana sylvestris | L-glutamate + oxaloacetate | - |
r | |
| 2-oxoglutarate + L-glutamate | - |
Nicotiana sylvestris | L-glutamate + 2-oxoglutarate | - |
r | |
| additional information | substrate specificity, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and indolepyruvate using cosubstrate L-glutamate, no activity with 4-hydroxyphenylpyruvate, phenylpyruvate, and pyruvate using cosubstrate L-aspartate | Nicotiana sylvestris | ? | - |
? | |
| oxaloacetate + L-aspartate | - |
Nicotiana sylvestris | L-aspartate + oxaloacetate | - |
r | |
| oxaloacetate + L-glutamate | - |
Nicotiana sylvestris | L-aspartate + 2-oxoglutarate | - |
r | |
| prephenate + L-aspartate | - |
Nicotiana sylvestris | L-arogenate + oxaloacetate | - |
r | |
| prephenate + L-glutamate | important step in biosynthesis of L-tyrosine and L-phenylalanine | Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | - |
r | |
| prephenate + L-glutamate | optimal prephenate concentration is 0.5-1.0 mM | Nicotiana sylvestris | L-arogenate + 2-oxoglutarate | - |
r | |
| pyruvate + L-glutamate | - |
Nicotiana sylvestris | L-alanine + 2-oxoglutarate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AT-C | - |
Nicotiana sylvestris |
| prephenate aminotransferase | - |
Nicotiana sylvestris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Nicotiana sylvestris |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 70 | - |
Nicotiana sylvestris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
stable, at least for 17 min | Nicotiana sylvestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.25 | - |
- |
Nicotiana sylvestris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required, stabilizes the enzyme | Nicotiana sylvestris | |
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