General Stability | Organism |
---|---|
pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay | Sorghum bicolor x Sorghum sudanesis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
prephenate | pH 8.0 | Sorghum bicolor x Sorghum sudanesis | |
0.8 | - |
L-arogenate | pH 8.0 | Sorghum bicolor x Sorghum sudanesis | |
1.07 | - |
L-glutamate | pH 8.0 | Sorghum bicolor x Sorghum sudanesis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + L-glutamate | Sorghum bicolor x Sorghum sudanesis | important step in biosynthesis of L-tyrosine and L-phenylalanine | L-arogenate + 2-oxoglutarate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor x Sorghum sudanesis | - |
hybrid | - |
Purification (Comment) | Organism |
---|---|
partially by ion exchange chromatography | Sorghum bicolor x Sorghum sudanesis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | etiolated, 4-days-old | Sorghum bicolor x Sorghum sudanesis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Sorghum bicolor x Sorghum sudanesis |
additional information | - |
partially purified enzyme, activity of prephenate aminotransferase as a function of the concentration of prephenate and glutamate | Sorghum bicolor x Sorghum sudanesis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + L-glutamate | 10% of the activity with prephenate at 1.0 mM substrate concentration | Sorghum bicolor x Sorghum sudanesis | L-tyrosine + 2-oxoglutarate | - |
r | |
oxaloacetate + L-glutamate | 78% of the activity with prephenate at 1.0 mM substrate concentration | Sorghum bicolor x Sorghum sudanesis | L-aspartate + 2-oxoglutarate | - |
r | |
prephenate + L-aspartate | 20% of the activity with L-glutamate | Sorghum bicolor x Sorghum sudanesis | L-arogenate + oxaloacetate | - |
r | |
prephenate + L-glutamate | important step in biosynthesis of L-tyrosine and L-phenylalanine | Sorghum bicolor x Sorghum sudanesis | L-arogenate + 2-oxoglutarate | - |
r | |
prephenate + L-glutamate | enzyme shows high affinity and specificity for prephenate, similar reaction rate in both reaction directions | Sorghum bicolor x Sorghum sudanesis | L-arogenate + 2-oxoglutarate | - |
r | |
pyruvate + L-glutamate | 10% of the activity with prephenate at 1.0 mM substrate concentration | Sorghum bicolor x Sorghum sudanesis | L-alanine + 2-oxoglutarate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
prephenate aminotransferase | - |
Sorghum bicolor x Sorghum sudanesis |
prephenate: glutamate aminotransferase | - |
Sorghum bicolor x Sorghum sudanesis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Sorghum bicolor x Sorghum sudanesis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
95% remaining activity after 17 min | Sorghum bicolor x Sorghum sudanesis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sorghum bicolor x Sorghum sudanesis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | no activity below pH 6.0 and above pH 10.5 | Sorghum bicolor x Sorghum sudanesis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sorghum bicolor x Sorghum sudanesis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sorghum bicolor x Sorghum sudanesis | isoelectric focusing | - |
5.2 |