Cloned (Comment) | Organism |
---|---|
expression with His-tag | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K274A | 265fold reduction of kcat-value | Escherichia coli |
K274R | 640fold reduction of kcat-value | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate | transition-state analogue, competitive | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
chorismate | wild-type, cosubstrate L-glutamine, pH 8.5, 25°C | Escherichia coli | |
0.058 | - |
chorismate | wild-type, cosubstrate NH4+, pH 8.5, 25°C | Escherichia coli | |
0.097 | - |
chorismate | mutant K274A, cosubstrate NH4+, pH 8.5, 25°C | Escherichia coli | |
145 | - |
NH4+ | wild-type, pH 8.5, 25°C | Escherichia coli | |
169 | - |
NH4+ | mutant K274A, pH 8.5, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate | mechanism includes nucleophile addition to C2 of chorismate in an SN2'' process | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chorismate + L-glutamine | - |
Escherichia coli | 4-amino-4-deoxychorismate + L-glutamate | - |
? | |
chorismate + NH4+ | - |
Escherichia coli | 4-amino-4-deoxychorismate + H2O + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000098 | - |
chorismate | mutant K274R, cosubstrate NH4+, pH 8.5, 25°C | Escherichia coli | |
0.00083 | - |
chorismate | mutant K274R, cosubstrate L-glutamine, pH 8.5, 25°C | Escherichia coli | |
0.002 | - |
chorismate | mutant K274A, cosubstrate L-glutamine, pH 8.5, 25°C | Escherichia coli | |
0.026 | - |
chorismate | mutant K274A, cosubstrate NH4+, pH 8.5, 25°C | Escherichia coli | |
0.032 | - |
chorismate | wild-type, cosubstrate NH4+, pH 8.5, 25°C | Escherichia coli | |
0.53 | - |
chorismate | wild-type, cosubstrate L-glutamine, pH 8.5, 25°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate | mutant K274A, pH 8.5, 25°C | Escherichia coli | |
0.0056 | - |
(5R,6R)-4-ammonio-5-((1-carboxylatovinyl)oxy)-6-hydroxycyclohex-1-ene-1-carboxylate | wild-type, pH 8.5, 25°C | Escherichia coli |