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Literature summary for 2.6.1.90 extracted from

  • Pfoestl, A.; Hofinger, A.; Kosma, P.; Messner, P.
    Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose in Aneurinibacillus thermoaerophilus L420-91T (2003), J. Biol. Chem., 278, 26410-26417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Aneurinibacillus thermoaerophilus

General Stability

General Stability Organism
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol Aneurinibacillus thermoaerophilus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metal ions are not required Aneurinibacillus thermoaerophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate Aneurinibacillus thermoaerophilus the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?

Organism

Organism UniProt Comment Textmining
Aneurinibacillus thermoaerophilus Q6T1W6
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-
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 Q6T1W6
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-

Purification (Commentary)

Purification (Comment) Organism
-
Aneurinibacillus thermoaerophilus

Reaction

Reaction Comment Organism Reaction ID
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate = dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate the reaction occurs in the reverse direction Aneurinibacillus thermoaerophilus

Storage Stability

Storage Stability Organism
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity Aneurinibacillus thermoaerophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate
-
Aneurinibacillus thermoaerophilus dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway Aneurinibacillus thermoaerophilus dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate
-
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate the glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtB is involved in this pathway Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate
-
?

Synonyms

Synonyms Comment Organism
FdtB
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Aneurinibacillus thermoaerophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aneurinibacillus thermoaerophilus
50 55
-
Aneurinibacillus thermoaerophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Aneurinibacillus thermoaerophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Aneurinibacillus thermoaerophilus