Application | Comment | Organism |
---|---|---|
synthesis | synthesis of TDP-D-ravidosamine, necessary for future in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities | Streptomyces ravidus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces ravidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41710 | - |
2 * 41710, SDS-PAGE | Streptomyces ravidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | Streptomyces ravidus | the enzyme is involved in the conversion of D-glucose-1-phosphate to dTDP-D-ravidosamine | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces ravidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces ravidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | - |
Streptomyces ravidus | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? | |
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose + L-glutamate | the enzyme is involved in the conversion of D-glucose-1-phosphate to dTDP-D-ravidosamine | Streptomyces ravidus | dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose + 2-oxoglutarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41710, SDS-PAGE | Streptomyces ravidus |
Synonyms | Comment | Organism |
---|---|---|
RavAMT | - |
Streptomyces ravidus |
TDP-3-keto-6-deoxy-D-galactose-3-aminotransferase | - |
Streptomyces ravidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces ravidus |
50 | 55 | - |
Streptomyces ravidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptomyces ravidus |