Literature summary for 2.6.1.90 extracted from
Pandey, R.; Parajuli, P.; Chu, L.; Darsandhari, S.; Sohng, J.
Biosynthesis of amino deoxy-sugar-conjugated flavonol glycosides by engineered Escherichia coli (2015), Biochem. Eng. J., 101, 191-199.
No PubMed abstract available
Application
Application |
Comment |
Organism |
synthesis |
engineering of Escherichia coli to divert the flow of carbon flux from glucose-1-phosphate to thymidine diphosphate 4-oxo 6-deoxy-D-glucose (dTKDG), an intermediate of various dTDP-sugars. Glucose phosphate isomerase, glucose-6-phosphate dehydrogenase and uridylyltransferase genes are deleted while dTDP-D-glucose synthase and dTDP-D-glucose 4,6-dehydratase are overexpressed to produce a pool of dTKDG in the cell cytosol. The flow of dTKDG is further diverted to dTDP-D-viosamine, dTDP 4-amino 4,6-dideoxy-D-galactose, and dTDP 3-amino 3,6-dideoxy-D-galactose sugars using sugar aminotransferases gerB, wecE, and fdtB, respectively, from different sources. These sugar moieties are transferred to the 3-hydroxyl position of quercetin and kaempferol with the help of Arabidopsis thaliana glycosyltransferase ArGT3 |
Aneurinibacillus thermoaerophilus |
Organism
Organism |
UniProt |
Comment |
Textmining |
Aneurinibacillus thermoaerophilus |
Q6T1W6 |
- |
- |
Synonyms
Synonyms |
Comment |
Organism |
FdtB |
- |
Aneurinibacillus thermoaerophilus |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
additional information |
enzyme utilizes an oxidized quinone as co-factor |
Aneurinibacillus thermoaerophilus |
|