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Literature summary for 2.6.1.97 extracted from

  • Phillips, G.; Swairjo, M.A.; Gaston, K.W.; Bailly, M.; Limbach, P.A.; Iwata-Reuyl, D.; de Crecy-Lagard, V.
    Diversity of archaeosine synthesis in crenarchaeota (2012), ACS Chem. Biol., 7, 300-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Sulfurisphaera tokodaii
-
L-glutamate + archaeine15 in tRNA i.e. G+, whcih is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Euryarchaeota
-
L-glutamate + archaeine15 in tRNA i.e. G+, which is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?

Organism

Organism UniProt Comment Textmining
Euryarchaeota
-
overview, gene arcS
-
no activity in Pyrobaculum calidifontis
-
strain JCM 11548
-
no activity in Pyrobaculum islandicum
-
-
-
no activity in Sulfolobus acidocaldarius
-
-
-
Saccharolobus solfataricus Q981C9
-
-
Saccharolobus solfataricus P2 Q981C9
-
-
Sulfurisphaera tokodaii
-
gene arcS
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
Sulfurisphaera tokodaii L-glutamate + archaeine15 in tRNA i.e. G+, whcih is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
Euryarchaeota L-glutamate + archaeine15 in tRNA i.e. G+, which is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O i.e. preQ0-tRNA Sulfurisphaera tokodaii L-glutamate + archaeine15 in tRNA
-
?
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O i.e. preQ0-tRNA Euryarchaeota L-glutamate + archaeine15 in tRNA
-
?
additional information in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016 Saccharolobus solfataricus ?
-
?
additional information in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016 Saccharolobus solfataricus P2 ?
-
?

Synonyms

Synonyms Comment Organism
ARChaeosine synthase
-
Sulfurisphaera tokodaii
ARChaeosine synthase
-
Euryarchaeota
ArcS
-
Sulfurisphaera tokodaii
ArcS
-
Euryarchaeota
ATP-independent amidinotransferase
-
Sulfurisphaera tokodaii
ATP-independent amidinotransferase
-
Euryarchaeota
SSO0016 locus name Saccharolobus solfataricus

General Information

General Information Comment Organism
evolution specific Archaea such as Sulfolobus tokodaii have retained ArcS in addition to GAT-QueC, overview Sulfurisphaera tokodaii
metabolism GAT-QueC also catalyzes biosynthesis of G+-tRNA, pathways, overview Sulfurisphaera tokodaii
additional information G+ can be tolerated in Escherichia coli at position 34 in normally Q-containing tRNA when recombinant GAT-QueC and QueF are introduced Euryarchaeota
physiological function ArcS catalyzes the final step in the G+ pathway, the conversion of preQ0-tRNA to G+-tRNA, in Euryarchaeota Sulfurisphaera tokodaii
physiological function ArcS catalyzes the final step in the G+ pathway, the conversion of preQ0-tRNA to G+-tRNA, in Euryarchaeota Euryarchaeota