Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of wei8 knockout mutants and of wei8-1 and tar2-1 mutants. If TAA1/TARs and YUCs work together to produce indole 3-acetic acid, blocking the in vivo activity of TAA1/TARs with L-kynurenine suppresses the high-auxin phenotypes of YUC1ox plants. The plants expressing the YUC1 cDNA under the control of the TAA1 regulatory sequences display a much stronger root phenotype, characterized by an extremely short root length both in light- and dark-grown seedlings. Significantly lower levels of free for indole 3-acetic acid are detected in the single wei8-2 as well as in the double wei8-2 tar2-1 mutants | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-kynurenine | a potent inhibitor of in vivo TAA1/TAR activity | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | Arabidopsis thaliana | - |
indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | Arabidopsis thaliana Col-0 | - |
indole-3-pyruvate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9S7N2 | gene wei8 | - |
Arabidopsis thaliana Col-0 | Q9S7N2 | gene wei8 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + pyruvate | - |
Arabidopsis thaliana | indole-3-pyruvate + L-alanine | - |
? | |
L-tryptophan + pyruvate | - |
Arabidopsis thaliana Col-0 | indole-3-pyruvate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tryptophan aminotransferase of Arabidopsis 1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | significantly lower levels of free for indole 3-acetic acid are detected in the single wei8-2 as well as in the double wei8-2 tar2-1 mutants | Arabidopsis thaliana |
metabolism | the three gene families, cytochrome P450 79B2/B3, YUCCA or YUC, and tryptophan aminotransferase of Arabidopsis 1/tryptopan aminotransferase related, i.e. TAA1/TAR, in the production of this hormone in the reference plant Arabidopsis thaliana. Each of these three gene families is believed to represent independent routes of auxin biosynthesis. TAA1/TARs and YUCs function in a common linear biosynthetic pathway that is genetically distinct from the CYP79B2/B3 route. In the redefined TAA1-YUC auxin biosynthetic pathway, TAA1/TARs are required for the production of indole-3-pyruvic acid from Trp, whereas YUCs are likely to function downstream, function of TAA1 and TARs is required for indole 3-acetic acid production via YUCs. Enzymatic reactions involved in indole-3-acetic acid production via indole-3-pyruvic acid, overview. The CYP79B2/B3 function is not required for YUC1-mediated auxin production | Arabidopsis thaliana |
additional information | if TAA1/TARs and YUCs work together to produce indole 3-acetic acid, blocking the in vivo activity of TAA1/TARs with L-kynurenine suppresses the high-auxin phenotypes of YUC1ox plants | Arabidopsis thaliana |