Cloned (Comment) | Organism |
---|---|
His-tagged protein and maltose binding protein fusion protein expressed in different Escherichia coli (DE3) strains at different temeratures and different expression levels | Sphingopyxis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), maltose binding protein fusion protein: affinity chromatography (dextrin sepharose), cleavage of the fusion protein | Sphingopyxis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S,5R,10S,12R,14R,15S,16S)-2-amino-12,16-dimethylicosane-3,5,10,14,15-pentol + pyruvate | - |
Sphingopyxis sp. | (3S,5R,10S,12R,14R,15S,16S)-3,5,10,14,15-pentahydroxy-12,16-dimethylicosan-2-one + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminotransferase for deamination of hydrolyzed fumonisin B1 (gene fumI) | - |
Sphingopyxis sp. |
FumI | - |
Sphingopyxis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sphingopyxis sp. |