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Literature summary for 2.7.1.100 extracted from
- Functional analysis of methylthioribose kinase genes in plants (2004), Plant Physiol., 136, 4061-4071.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Arabidopsis thaliana |
| DNA and amino acid sequence determination and analysis, overvexpression of a slightly modified enzyme in Escherichia coli strain BL21 as GST-fusion protein | Oryza sativa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | insertional mutagenesis results in inability of the plants to grow on methylthioadenosine as asupplemntal sulfur source, knockout plants show unimpaired growth under standard conditions | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | cooperative kinetics | Oryza sativa | |
| 0.0168 | - |
S-methyl-5-thio-D-ribose | pH 8.5, 37°C | Oryza sativa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Oryza sativa | |
| Mg2+ | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48600 | - |
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
| 50000 | - |
x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + S-methyl-5-thio-D-ribose | Oryza sativa | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| ATP + S-methyl-5-thio-D-ribose | Arabidopsis thaliana | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| additional information | Oryza sativa | the enzyme regulation is related to the sulfur metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9C6D2 | - |
- |
| Oryza sativa | Q7XR61 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off | Oryza sativa |
| recombinant GST-fusion protein from Escherichia coli by glutathione affinity chromatography, the GST-tag is cleaved off | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| root | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa | - |
| shoot | strong upregulation of enzyme expression during sulfur starvation | Oryza sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + S-methyl-5-thio-D-ribose | - |
Oryza sativa | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| ATP + S-methyl-5-thio-D-ribose | - |
Arabidopsis thaliana | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| ATP + S-methyl-5-thio-D-ribose | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | Oryza sativa | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| ATP + S-methyl-5-thio-D-ribose | enzyme is involved in the recycling of methionine through the methylthioadenosine, i.e. MTA, cycle | Arabidopsis thaliana | ADP + S-methyl-5-thio-D-ribose 1-phosphate | - |
? | |
| additional information | the enzyme regulation is related to the sulfur metabolism | Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48600, SDS-PAGE, x * 50000, recombinant enzyme, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methylthioribose kinase | - |
Oryza sativa |
| methylthioribose kinase | - |
Arabidopsis thaliana |
| MTK | - |
Arabidopsis thaliana |
| MTK1 | - |
Oryza sativa |
| MTR kinase | - |
Arabidopsis thaliana |
| MTR kinase1 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryza sativa |
| 37 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Oryza sativa |
| 8.5 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Oryza sativa | |
| ATP | - |
Arabidopsis thaliana | |
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