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Literature summary for 2.7.1.105 extracted from
- Characterization of 6-phosphofructo-2-kinase from foetal-rat liver (1992), Biochem. J., 281, 457-463.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; phosphorylation of foetal liver enzyme by protein kinase C, but no effect on adult liver cells | Rattus norvegicus | |
| sn-glycerol 3-phosphate | stimulates phosphatase activity | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.044 | - |
beta-D-fructose 6-phosphate | pH 8.5, 30°C, foetal liver enzyme | Rattus norvegicus | |
| 0.047 | - |
beta-D-fructose 6-phosphate | pH 8.5, 30°C, adult liver enzyme | Rattus norvegicus | |
| 0.19 | - |
ATP | pH 8.5, 30°C, foetal enzyme | Rattus norvegicus | |
| 0.195 | - |
ATP | pH 8.5, 30°C, adult enzyme | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | MgATP2 | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
2 * 55000, SDS-PAGE | Rattus norvegicus |
| 89100 | - |
foetal liver, gel filtration | Rattus norvegicus |
| 100000 | - |
adult liver, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
foetal and adult | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylation of foetal liver enzyme by protein kinase C, but no effect on adult liver cells | Rattus norvegicus |
| phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.055 | - |
adult | Rattus norvegicus |
| 0.08 | - |
foetal | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
sn-glycerol 3-phosphate | pH 7.1 | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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