Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | heart enzyme is more sensitive than liver enzyme | Bos taurus | |
citrate | heart enzyme is more sensitive than liver enzyme | Rattus norvegicus | |
additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme | Bos taurus | |
additional information | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme; rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme | Rattus norvegicus | |
sn-glycerol 3-phosphate | heart enzyme is less sensitive than liver | Bos taurus | |
sn-glycerol 3-phosphate | heart enzyme is less sensitive than liver | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
beta-D-fructose 6-phosphate | - |
Rattus norvegicus | |
0.05 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C | Bos taurus | |
0.1 | - |
ATP | pH 7.5, 30°C, MgATP | Rattus norvegicus | |
0.1 | - |
ATP | pH 7.5, 30°C, MgATP | Bos taurus | |
0.14 | - |
beta-D-fructose 6-phosphate | pH 7.5, 30°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgATP2 | Rattus norvegicus | |
Mg2+ | MgATP2 | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Bos taurus |
58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
97000 | - |
gel filtration | Bos taurus |
112500 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | Rattus norvegicus | responsible for regulation of fructose 2,6-bisphosphate-concentration | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | Bos taurus | responsible for regulation of fructose 2,6-bisphosphate-concentration | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Rattus norvegicus |
phosphoprotein | phosphorylation by cAMP-dependent protein kinase causes inactivation of liver enzyme | Bos taurus |
phosphoprotein | rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme | Rattus norvegicus |
phosphoprotein | rat liver enzyme is inhibited by phosphorylation by cAMP-dependent protein kinase, but not rat skeletal muscle, bovine and rat heart enzyme | Bos taurus |
Purification (Comment) | Organism |
---|---|
heart | Bos taurus |
liver | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus | |
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
heart | - |
Bos taurus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
ATP + beta-D-fructose 6-phosphate | - |
Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
ATP + beta-D-fructose 6-phosphate | responsible for regulation of fructose 2,6-bisphosphate-concentration | Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
ATP + beta-D-fructose 6-phosphate | responsible for regulation of fructose 2,6-bisphosphate-concentration | Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? | |
additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 52000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Bos taurus |
7.1 | - |
- |
Rattus norvegicus |