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Literature summary for 2.7.1.105 extracted from
- Isolation and characterization of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from bovine liver (1985), Arch. Biochem. Biophys., 238, 531-543.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sn-glycerol 3-phosphate | i.e. alpha-glycerol phosphate | Bos taurus |  |
| sn-glycerol 3-phosphate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | 0.012 | MgATP2- | pH 7.5, 30°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | MgATP2 | Rattus norvegicus | |
| Mg2+ | MgATP2 | Bos taurus | |
| phosphate | - |
Rattus norvegicus | |
| phosphate | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
2 * 49000, SDS-PAGE | Bos taurus |
| 55000 | - |
2 * 55000, SDS-PAGE | Rattus norvegicus |
| 102000 | - |
gel filtration | Bos taurus |
| 110000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus | |
| ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.042 | - |
- |
Bos taurus |
| 0.06 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | via phosphorylenzyme intermediate | r | |
| ATP + beta-D-fructose 6-phosphate | - |
Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | via phosphorylenzyme intermediate | r | |
| MgATP2- + beta-D-fructose 6-phosphate | - |
Bos taurus | ? | - |
? | |
| additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? | |
| additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 55000, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 49000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
rat | Rattus norvegicus |
| 8.5 | - |
- |
Rattus norvegicus |
| 8.5 | - |
bovine | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 9.5 | about half-maximal activity at pH 7.3 and 9.5 | Bos taurus |
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