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Literature summary for 2.7.1.105 extracted from
- Purification and properties of spinach leaf phosphofructokinase 2/fructose 2,6-bisphosphatase [published erratum appears in Eur J Biochem 1987 Feb 2;162(3):699] (1986), Eur. J. Biochem., 161, 351-357.
General Stability
| General Stability | Organism |
|---|---|
| unstable in crude homogenate, iodoacetate, antipain, chymostatin or leupeptin stabilizes, not pepstatin, PMSF or soybean trypsin inhibitor | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dihydroxyacetone phosphate | weak | Spinacia oleracea |  |
| diphosphate | - |
Spinacia oleracea | |
| glycerate 2-phosphate | - |
Spinacia oleracea | |
| glycolate 2-phosphate | - |
Spinacia oleracea | |
| additional information | phosphorylation by cAMP-dependent protein kinase, but no inhibition | Ricinus communis | |
| additional information | - |
Spinacia oleracea | |
| phosphoenolpyruvate | - |
Spinacia oleracea | |
| sn-glycerol 3-phosphate | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data of native and phosphorylated enzyme | Spinacia oleracea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Spinacia oleracea | |
| phosphate | activation | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
4 * 90000, H-form, SDS-PAGE | Spinacia oleracea |
| 90000 | - |
4 * 90000, H-form, SDS-PAGE | Ricinus communis |
| 90000 | - |
4 * 90000, H-form, SDS-PAGE | Helianthus tuberosus |
| 132000 | - |
L-form, gel filtration | Spinacia oleracea |
| 390000 | - |
H-form, gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus tuberosus | - |
Jerusalem artichoke | - |
| Ricinus communis | - |
castor bean | - |
| Spinacia oleracea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Spinacia oleracea |
| phosphoprotein | phosphorylation by cAMP-dependent protein kinase, but no inhibition | Ricinus communis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| multiple molecular forms, predominant: H-(heavy) and L-(light)form | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Ricinus communis | - |
| leaf | - |
Spinacia oleracea | - |
| tuber | - |
Helianthus tuberosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.718 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, in 100 mM potassium acetate, 5 mM magnesium acetate, 2.5 mM DTT, 1 mM iodoacetate, 1 mg/ml antipain, 50 mM Tris-acetate, pH 7.8, several h | Spinacia oleracea |
| 0°C, L-form, at least 2 h in the absence of protease inhibitor | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Spinacia oleracea | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| ATP + beta-D-fructose 6-phosphate | - |
Ricinus communis | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| ATP + beta-D-fructose 6-phosphate | - |
Helianthus tuberosus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 90000, H-form, SDS-PAGE | Spinacia oleracea |
| tetramer | 4 * 90000, H-form, SDS-PAGE | Ricinus communis |
| tetramer | 4 * 90000, H-form, SDS-PAGE | Helianthus tuberosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Spinacia oleracea |
| 30 | - |
assay at | Ricinus communis |
| 30 | - |
assay at | Helianthus tuberosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Spinacia oleracea |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
glycerate 2-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.018 | - |
phosphoenolpyruvate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.018 | - |
sn-glycerol 3-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.02 | - |
glycolate 2-phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.026 | - |
diphosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.14 | - |
glycerate 2-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.15 | - |
phosphoenolpyruvate | H form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.16 | - |
diphosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.16 | - |
sn-glycerol 3-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.18 | - |
glycolate 2-phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
| 0.25 | - |
dihydroxyacetone phosphate | L form, pH 7.8, 30°C | Spinacia oleracea | |
| 1.9 | - |
dihydroxyacetone phosphate | H form, pH 7.8, 30°C | Spinacia oleracea | |
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