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Literature summary for 2.7.1.105 extracted from

  • Watanabe, F.; Jameson, D.M.; Uyeda, K.
    Enzymic and fluorescence studies of four single-tryptophan mutants of rat testis fructose 6-phosphate, 2-kinase:fructose 2,6-bisphosphatase (1996), Protein Sci., 5, 904-913.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.105

Protein Variants

Protein Variants Comment Organism
additional information enzyme contains four tryptophan residues: Trp15, Trp64, Trp299 and Trp320, mutant enzymes contain either no Trp or a single Trp at each location, the other Trp residues having been converted to Phe, mutations do not cause large change in protein conformation and function, Trp299 and Trp320 in mutant enzymes quenched by iodide to a small extent can indicate an altered conformation, Trp64 is not accessible Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
guanidine inactivation, unfolding Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information comparison of Km of wild-type and mutant enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 r
additional information also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) Rattus norvegicus ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information guanidine-inhibition of wild-type and mutant enzymes Rattus norvegicus