Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Insulin | activation of transfected heart enzyme in human embryonic kidney 293 cells by inducing the phosphorylation of Ser466 and Ser483, activation is mediated by 3-phosphoinositide-dependent kinase-1, protein kinase B is in vivo not essential, activation is sensitive to LY294002 and wortmannin and insensitive to rapaycin and PD98059 | Bos taurus |
Cloned (Comment) | Organism |
---|---|
wild-type enzyme, S466E and S466E/S483E mutants are expressed in Escherichia coli BL21(DE3)pLysE and S483E mutant is expressed in BL21(DE3)pLysS, human embryonic kidney 293 cells are transiently transfected with vectors expressing wild-type heart enzyme | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
S466E | mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme in vitro | Bos taurus |
S466E/S483E | the double mutant is not phosphorylated by protein kinase B, mutation decreases Km of beta-D-fructose 6-phosphate in vitro | Bos taurus |
S483E | mutant is phosphorylated by protein kinase B with a stoichiometry to about half that of wild-type enzyme and mutation decreases citrate inhibition in vitro | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Km of wild-type and mutant enzyme | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzyme | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | - |
Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Bos taurus |