Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E190Q | mutant presents a 50fold decrease in the kcat value and a 15fold increment in the apparent Km for MgATP2+. E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgATP2- | E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate | Escherichia coli | |
phosphate | inhibits wild-type and mutant enzym E190Q. E190 contributs to the mechanism of phosphate inhibition in Pfk-2. E190Q mutant presents alterations in the inhibition by MgATP2- and phosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0094 | - |
D-fructose 6-phosphate | mutant enzyme E190Q, 30 mM Mg2+ | Escherichia coli | |
0.01 | - |
D-fructose 6-phosphate | mutant enzyme E190Q, 1 mM Mg2+ | Escherichia coli | |
0.02 | - |
MgATP2- | wild-type enzyme, 1 mM Mg2+ | Escherichia coli | |
0.022 | - |
MgATP2- | wild-type enzyme, 30 mM Mg2+ | Escherichia coli | |
0.028 | - |
D-fructose 6-phosphate | wild-type enzyme, 30 mM Mg2+ | Escherichia coli | |
0.1 | - |
D-fructose 6-phosphate | wild-type enzyme, 1 mM Mg2+ | Escherichia coli | |
0.149 | - |
MgATP2- | mutant enzyme E190Q, 1 mM Mg2+ | Escherichia coli | |
0.32 | - |
MgATP2- | mutant enzyme E190Q, 30 mM Mg2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement of a Mg2+ ion, besides the one present in the metal-nucleotide complex, for catalysis in the wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 2,6-bisphosphate | - |
? | |
MgATP2- + beta-D-fructose 6-phosphate | - |
Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Pfk-2 | - |
Escherichia coli |
phosphofructokinase-2 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
D-fructose 6-phosphate | mutant enzyme E190Q, 1 mM Mg2+ | Escherichia coli | |
0.14 | - |
MgATP2- | mutant enzyme E190Q, 1 mM Mg2+ | Escherichia coli | |
1.43 | - |
D-fructose 6-phosphate | mutant enzyme E190Q, 30 mM Mg2+ | Escherichia coli | |
1.43 | - |
MgATP2- | mutant enzyme E190Q, 30 mM Mg2+ | Escherichia coli | |
53 | - |
D-fructose 6-phosphate | wild-type enzyme, 1 mM Mg2+ | Escherichia coli | |
53 | - |
MgATP2- | wild-type enzyme, 1 mM Mg2+ | Escherichia coli | |
53 | - |
D-fructose 6-phosphate | wild-type enzyme, 30 mM Mg2+ | Escherichia coli | |
53 | - |
MgATP2- | wild-type enzyme, 30 mM Mg2+ | Escherichia coli |