Crystallization (Comment) | Organism |
---|---|
sitting-drop, vapor-diffusion method, crystal structures of PFKFB3/beta,gamma-methylene-adenosine 5'-triphosphate/fructose-6-phosphate and PFKFB3/ADP/phosphoenolpyruvate complexes are determined to 2.7 A and 2.25 A resolution | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
A44G | the Km values for ATP and fructose-6-phosphate of the mutant enzyme are decreased by approximately 20fold compared to wilde-type values | Rattus norvegicus |
A44V | the Km values for ATP and fructose-6-phosphate of the mutant enzyme are decreased by 8fold and 20fold compared to wilde-type values | Rattus norvegicus |
L148N | inactive mutant enzyme | Rattus norvegicus |
L168A | inactive mutant enzyme | Rattus norvegicus |
L168R | mutant enzyme shows 0.2% of wild-type activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00087 | - |
ATP | mutant enzyme A44G | Rattus norvegicus | |
0.0038 | - |
beta-D-fructose 6-phosphate | mutant enzyme A44G | Rattus norvegicus | |
0.0102 | - |
beta-D-fructose 6-phosphate | wild-type enzyme | Rattus norvegicus | |
0.0169 | - |
ATP | wild-type enzyme | Rattus norvegicus | |
0.14 | - |
ATP | mutant enzyme A44V | Rattus norvegicus | |
0.204 | - |
beta-D-fructose 6-phosphate | mutant enzyme A44V | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-D-fructose 6-phosphate | site-directed mutant study and inhibition kinetics suggest that the reaction will be catalyzed most efficiently by the protein when the substrates bind to the active pocket in an ordered manner in which ATP binds first | Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase | bifunctional enzyme | Rattus norvegicus |
PFKFB3 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
ATP | wild-type enzyme | Rattus norvegicus | |
0.14 | - |
beta-D-fructose 6-phosphate | wild-type enzyme | Rattus norvegicus | |
0.17 | - |
ATP | mutant enzyme A44V | Rattus norvegicus | |
0.17 | - |
beta-D-fructose 6-phosphate | mutant enzyme A44V | Rattus norvegicus | |
0.19 | - |
ATP | mutant enzyme A44G | Rattus norvegicus | |
0.19 | - |
beta-D-fructose 6-phosphate | mutant enzyme A44G | Rattus norvegicus |