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Literature summary for 2.7.1.105 extracted from
- Islet fructose 6-phosphate, 2-kinase: fructose 2,6-bisphosphatase: isozymic form, expression, and characterization (1996), Biochem. Biophys. Res. Commun., 218, 159-163.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| citrate | - |
Bos taurus |  |
| citrate | - |
Rattus norvegicus | |
| additional information | - |
Bos taurus | |
| additional information | the islet enzyme lacks protein kinase A and C phosphorylation sites | Rattus norvegicus | |
| phosphoenolpyruvate | - |
Bos taurus | |
| phosphoenolpyruvate | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, liver | Rattus norvegicus | |
| 0.017 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, islet | Rattus norvegicus | |
| 0.023 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, heart | Bos taurus | |
| 0.04 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, testis | Rattus norvegicus | |
| 0.048 | - |
ATP | pH 7.5, 25°C, heart | Rattus norvegicus | |
| 0.056 | - |
beta-D-fructose 6-phosphate | pH 7.5, 25°C, muscle | Rattus norvegicus | |
| 0.083 | - |
ATP | pH 7.5, 25°C, heart | Bos taurus | |
| 0.1 | - |
ATP | - |
Bos taurus | |
| 0.1 | - |
ATP | pH 7.5, 25°C, testis | Rattus norvegicus | |
| 0.31 | - |
ATP | pH 7.5, 25°C, liver | Rattus norvegicus | |
| 0.328 | - |
ATP | pH 7.5, 25°C, islet | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | Rattus norvegicus | responsible for regulation of fructose 2,6-bisphosphate-concentration | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| ATP + beta-D-fructose 6-phosphate | Bos taurus | responsible for regulation of fructose 2,6-bisphosphate-concentration | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Bos taurus |
| phosphoprotein | the islet enzyme lacks protein kinase A and C phosphorylation sites | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Rattus norvegicus | |
| ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | bifunctional protein: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, reverse reaction catalysed by fructose 2,6-bisphosphatase: 3.1.3.46 | Bos taurus | |
| ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate | high 6-phosphofructo-2-kinase activity compared to the fructose 2,6-bisphosphatase | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
| heart | - |
Bos taurus | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
| pancreatic islet | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-D-fructose 6-phosphate | - |
Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
| ATP + beta-D-fructose 6-phosphate | - |
Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
r | |
| ATP + beta-D-fructose 6-phosphate | responsible for regulation of fructose 2,6-bisphosphate-concentration | Rattus norvegicus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| ATP + beta-D-fructose 6-phosphate | responsible for regulation of fructose 2,6-bisphosphate-concentration | Bos taurus | ADP + beta-D-fructose 2,6-bisphosphate | - |
? | |
| additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Rattus norvegicus | ? | - |
? | |
| additional information | also catalyses the degradation of fructose 2,6-bisphosphate (EC 3.1.3.46) | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Rattus norvegicus |
| 25 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
phosphoenolpyruvate | pH 7.5, 25°C, islet | Rattus norvegicus | |
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