Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cholesterol 3-sulfate | activates | Escherichia coli | |
hexadecylphosphorylcholine | activates | Escherichia coli | |
Sodium dodecyl sulfate | activates | Escherichia coli | |
Triton X-100 | activates | Escherichia coli |
General Stability | Organism |
---|---|
when cosolubilized with diacylglycerol in octylglucoside micelles, the enzyme undergoes rapid irreversible inactivation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli | |
diacylglycerol | above 3.4 mol%, substrate inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for dioleoylglycerol is 0.92 mol% | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | tightly associated with the inner membrane | Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | enzyme requires a free divalent metal cation: Mg2+, Mn2+, Co2+, Cd2+ or Zn2+ | Escherichia coli | |
Co2+ | enzyme requires a free divalent metal cation: Mg2+, Mn2+, Co2+, Cd2+ or Zn2+ | Escherichia coli | |
Mg2+ | enzyme requires a free divalent metal cation: Mg2+, Mn2+, Co2+, Cd2+ or Zn2+ | Escherichia coli | |
Mn2+ | enzyme requires a free divalent metal cation: Mg2+, Mn2+, Co2+, Cd2+ or Zn2+. Ka for Mg2+ is 3.4 mM | Escherichia coli | |
Zn2+ | enzyme requires a free divalent metal cation: Mg2+, Mn2+, Co2+, Cd2+ or Zn2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | Escherichia coli | the enzyme functions to recycle diacylglycerol which is generated largely as a by-product of membrane-derived oligosaccharide biosynthesis | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-diacyl-sn-glycerol | the enzyme functions to recycle diacylglycerol which is generated largely as a by-product of membrane-derived oligosaccharide biosynthesis | Escherichia coli | ADP + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
ATP + ceramide | - |
Escherichia coli | ADP + ceramide 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Escherichia coli | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
addition of phospholipids provides some protection from thermal inactivation | Escherichia coli |
57 | - |
Triton-solubilized preparation, t1/2: 12 min | Escherichia coli |
100 | - |
enzyme activity of membranes, t1/2: 20 min | Escherichia coli |