Activating Compound | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine. Diacylglycerol analogs containing an unsaturated fatty acid at the sn-2 position give optimal enzyme activity irrespective of the presence of deoxycholate | Homo sapiens | |
phosphatidylcholine | - |
Escherichia coli | |
phosphatidylcholine | the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine | Homo sapiens | |
phosphatidylserine | - |
Escherichia coli | |
phosphatidylserine | the enzyme shows optimal activity in presence of phosphatidylserine or deoxycholate. Lower activity in presence of phosphatidylcholine | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
subcloning into the expression vector pMT-2 and transfection in COS-7 cells results in a 6-7fold increase in diacylglycerol kinase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
1,2-diolein | pH 7.5, 30°C | Homo sapiens | |
0.5 | - |
ATP | pH 7.5, 30°C, without deoxycholate | Homo sapiens | |
1.6 | - |
ATP | pH 7.5, 30°C, with 1 mM deoxycholate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
membrane | bound to | Escherichia coli | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Homo sapiens | - |
T-lymphocyte | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.913 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1,2-dipalmitoyl-sn-glycerol | 15% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | ADP + 1,2-dipalmitoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-O-hexadecyl-2-oleoyl-sn-glycerol | 45.4% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | ADP + 1-O-hexadecyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-palmitoyl-2-oleoyl-sn-glycerol | 96.5% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | ADP + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol | since diacylglycerol kinase is an enzyme of the phosphatidylinositol cycle, its natural substrate could be 1-stearoyl-2-arachidonoyl-sn-glycerol, thought to be the main diacylglycerol analog generated from phosphoinositide | Homo sapiens | ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
ATP + 2,3-dioleoyl-sn-glycerol | - |
Homo sapiens | ADP + 2,3-diacyl-sn-glycerol 1-phosphate | - |
? | |
ATP + 2-monooleoyl-rac-glycerol | 10.7% of the activity with 1-stearoyl-2-arachidonoyl-sn-glycerol | Homo sapiens | ADP + 2-monooleoyl-rac-glycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Escherichia coli | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? | |
ATP + sn-1,2-dioleoylglycerol | - |
Homo sapiens | ADP + sn-1,2-dioleoylglycerol 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Escherichia coli |
monomer | 1 * 86000, SDS-PAGE | Homo sapiens |