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Literature summary for 2.7.1.107 extracted from

  • Lau, F.W.; Chen, X.; Bowie, J.U.
    Active sites of diacylglycerol kinase from Escherichia coli are shared between subunits (1999), Biochemistry, 38, 5521-5527.
    View publication on PubMed
Search for more literature for 2.7.1.107

Protein Variants

Protein Variants Comment Organism
A14Q significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes, subunit mixing experiments of mutant enzymes Escherichia coli
D95N significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 18fold, subunit mixing experiments of mutant enzymes Escherichia coli
E69C mutant enzyme has an altered structure even in SDS Escherichia coli
E76L significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes Escherichia coli
K94L significantly impaired catalytic function, without evidence of gross structural alterations. Km-value for MgATP2- raises 13fold, subunit mixing experiments of mutant enzymes Escherichia coli
N72S significantly impaired catalytic function, without evidence of gross structural alterations, subunit mixing experiments of mutant enzymes Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
0.12
-
 ATP wild-type enzyme Escherichia coli
0.12
-
 ATP ATP in form of MgATP2- Escherichia coli
0.14
-
 ATP mutant enzyme E76L Escherichia coli
0.14
-
 ATP ATP in form of MgATP2- Escherichia coli
0.33
-
 ATP mutant enzyme E69C Escherichia coli
0.33
-
 ATP ATP in form of MgATP2- Escherichia coli
0.44
-
 ATP mutant enzyme N72S Escherichia coli
0.44
-
 ATP ATP in form of MgATP2- Escherichia coli
0.91
-
 ATP mutant enzyme A14Q Escherichia coli
0.91
-
 ATP ATP in form of MgATP2- Escherichia coli
1.5
-
 ATP mutant enzyme K94V Escherichia coli
1.5
-
 ATP ATP in form of MgATP2- Escherichia coli
2.1
-
 ATP mutant enzyme D95N Escherichia coli
2.1
-
 ATP ATP in form of MgATP2- Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1,2-diacyl-sn-glycerol
-
 Escherichia coli ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?