General Stability | Organism |
---|---|
glycerol and KCl or ATP stabilize | Escherichia coli |
glycerol and KCl or ATP stabilize | Solanum tuberosum |
glycerol and KCl or ATP stabilize | Solanum acaule |
glycerol and KCl or ATP stabilize | Solanum commersonii |
glycerol and KCl or ATP stabilize | Solanum megistacrolobum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli | |
Mg2+ | required for activity | Solanum tuberosum | |
Mg2+ | required for activity | Solanum acaule | |
Mg2+ | required for activity | Solanum commersonii | |
Mg2+ | required for activity | Solanum megistacrolobum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46300 | - |
x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea | Solanum tuberosum |
49500 | - |
x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea | Solanum tuberosum |
50000 | - |
x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea | Solanum tuberosum |
53000 | - |
x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea | Solanum tuberosum |
196000 | - |
isoform I, gel filtration | Solanum tuberosum |
201000 | - |
isoform II, gel filtration | Solanum tuberosum |
203000 | - |
isoform III, gel filtration | Solanum tuberosum |
800000 | - |
isoform IV, gel filtration | Solanum tuberosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Escherichia coli | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Solanum tuberosum | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Solanum acaule | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Solanum commersonii | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Solanum megistacrolobum | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | Escherichia coli DF1020 | first step in glycolysis | ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing overproducing plasmid pHE1007 | - |
Escherichia coli DF1020 | - |
containing overproducing plasmid pHE1007 | - |
Solanum acaule | - |
- |
- |
Solanum commersonii | - |
wild potato species | - |
Solanum megistacrolobum | - |
- |
- |
Solanum tuberosum | - |
potato, cv. Record, Hertha or Cara, isoforms I-IV | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
isoforms I-IV, DEAE-Sephacel, Red-agarose, ATP-agarose, Mono Q | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum tuberosum | - |
leaf | - |
Solanum acaule | - |
leaf | - |
Solanum commersonii | - |
leaf | - |
Solanum megistacrolobum | - |
tuber | - |
Solanum tuberosum | - |
tuber | - |
Solanum commersonii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
189 | 215 | - |
Escherichia coli |
200 | - |
isoforms I-IV | Solanum tuberosum |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks | Escherichia coli |
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks | Solanum tuberosum |
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks | Solanum acaule |
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks | Solanum commersonii |
-20°C, 0.1 M Tris-HCl buffer, pH 8, 2 mM MgCl2, 1 mM EDTA, 14 mM 2-mercaptoethanol, 10% v/v glycerol, 20% loss of activity within 3 weeks | Solanum megistacrolobum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Solanum tuberosum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Solanum acaule | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Solanum commersonii | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Solanum megistacrolobum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Solanum tuberosum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Solanum acaule | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Solanum commersonii | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Solanum megistacrolobum | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Escherichia coli DF1020 | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | first step in glycolysis | Escherichia coli DF1020 | ADP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | - |
Escherichia coli |
oligomer | - |
Solanum acaule |
oligomer | - |
Solanum commersonii |
oligomer | - |
Solanum megistacrolobum |
oligomer | x * 46300 + x * 49500 + x * 50000 + x * 53000, 4 enzyme forms of different subunit composition, SDS-PAGE with 4 M urea | Solanum tuberosum |