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Literature summary for 2.7.1.11 extracted from
- Phosphofructokinase from oyster adductor muscle (1982), Methods Enzymol., 90, 39-44.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | activation | Crassostrea virginica |  |
General Stability
| General Stability | Organism |
|---|---|
| polyethylene glycol stabilizes | Crassostrea virginica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arginine phosphate | - |
Crassostrea virginica | |
| citrate | cAMP, ADP or fructose 1,6-bisphosphate restore activity | Crassostrea virginica | |
| additional information | not inhibited by ADP; not inhibited by Ca2+; not inhibited by fructose 1,6-bisphosphate | Crassostrea virginica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
Mg2+ | pH 7.9, 24°C | Crassostrea virginica | |
| 0.05 | - |
ATP | pH 7.9, 24°C | Crassostrea virginica | |
| 0.5 | - |
D-fructose 6-phosphate | pH 7.9, 24°C | Crassostrea virginica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Crassostrea virginica | |
| Mg2+ | Km: 0.01 mM | Crassostrea virginica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 340000 | - |
- |
Crassostrea virginica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crassostrea virginica | - |
oyster | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| polyethylene glycol, ATP-agarose, Sephacryl S-300 | Crassostrea virginica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 146.7 | - |
- |
Crassostrea virginica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, t1/2: 1.5-2 days, ATP and/or fructose 1,6-bisphosphate increase stability | Crassostrea virginica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-fructose 6-phosphate | - |
Crassostrea virginica | ADP + D-fructose 1,6-bisphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Crassostrea virginica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Crassostrea virginica |
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Release 2023.1 (January 2023)
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