Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | isoenzyme PFK2 | Escherichia coli | |
phosphoenolpyruvate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
D-fructose 6-phosphate | pH 8.2, 28°C, PFK2 | Escherichia coli | |
0.05 | - |
ATP | pH 8.2, 28°C, PFK2 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Escherichia coli | |
Mg2+ | MgATP is the active substrate | Escherichia coli | |
Mn2+ | as effective as Mg2+ | Escherichia coli | |
Mn2+ | activation | Escherichia coli | |
Mn2+ | active substrate: MnATP2-, isoenzyme PFK2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, isoenzyme PFK1, tetrahedral arranged subunits, SDS-PAGE | Escherichia coli |
37000 | - |
2 * 37000, isoenzyme PFK2, SDS-PAGE | Escherichia coli |
37000 | - |
4 * 37000, isoenzyme PFK2, SDS-PAGE in the presence of 1 mM ATP, 5 mM Mg2+ | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K10 or K12 strains Hfr3000, DF1651, DF500, DF443, DF1651B1, AMIR20, PFK1 and PFK2 | - |
Purification (Comment) | Organism |
---|---|
PFK1, Blue Dextran, heat denaturation, PFK2, Sepharose-Blue Dextran, hydroxyapatite, ammonium sulfate, Blue Dextran, heat denaturation | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
190 | - |
isoenzyme PFK1 | Escherichia coli |
205 | - |
isoenzyme PFK2 | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, 65% saturated ammonium sulfate-suspension, at least 2 months, no loss of PFK1 activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | no activity with glucose 6-phosphate | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | best phosphoryl donors and acceptors of PFK1 and PFK2 | Escherichia coli | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-tagatose 6-phosphate | poor substrate for isoenzyme PFK2 | Escherichia coli | ADP + ? | - |
? | |
CTP + D-fructose 6-phosphate | less effective than ATP | Escherichia coli | CDP + D-fructose 1,6-bisphosphate | - |
? | |
CTP + D-fructose 6-phosphate | ITP, GTP or UTP as phosphoryl donors | Escherichia coli | CDP + D-fructose 1,6-bisphosphate | - |
? | |
dATP + D-fructose 6-phosphate | as good as ATP | Escherichia coli | dADP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | less effective than ATP or ITP, better than UTP or CTP | Escherichia coli | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | less effective than ATP | Escherichia coli | IDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | better than GTP, UTP or CTP | Escherichia coli | IDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | less effective than ATP, ITP, GTP, better than CTP | Escherichia coli | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, isoenzyme PFK2, SDS-PAGE | Escherichia coli |
More | in the absence of substrates PFK2 is a dimer, in the presence of high concentrations of ATP or ATP analogs, this dimer aggregates into a tetramer, aggregation is reversed by adddition of fructose 6-phosphate | Escherichia coli |
tetramer | 4 * 37000, isoenzyme PFK2, SDS-PAGE in the presence of 1 mM ATP, 5 mM Mg2+ | Escherichia coli |
tetramer | 4 * 35000, isoenzyme PFK1, tetrahedral arranged subunits, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 28 | assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
isoenzyme PFK2, pH-optima at pH 6.5 and pH 8.5 | Escherichia coli |
8.5 | - |
isoenzyme PFK2, pH-optima at pH 6.5 and pH 8.5 | Escherichia coli |