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Literature summary for 2.7.1.11 extracted from

  • Kemp, R.G.
    Phosphofructokinase from yeast (1975), Methods Enzymol., 42C, 71-77.
No PubMed abstract available
Search for more literature for 2.7.1.11

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Oryctolagus cuniculus

General Stability

General Stability Organism
dilution inactivates Oryctolagus cuniculus
very stable in Tris-phosphate or glycylglycine-glycerol buffer, pH 8, at 10-20 mg protein per ml Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
muscle PFK, isopropanol, heat treatment, ammonium sulfate, crystalization Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
 muscle PFK Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
4°C, crystalline, 6 years Oryctolagus cuniculus
very stable in Tris-phosphate or glycylglycine-glycerol buffer, pH 8, at high protein concentrations Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose 6-phosphate
-
 Oryctolagus cuniculus ADP + D-fructose 1,6-bisphosphate
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 below, stability is greatly diminished Oryctolagus cuniculus