Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GDP | activation at low concetrations, inhibition above 1 mM | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and G212V mutant PFK in Escherichia coli | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
G212V | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, native PFK | Geobacillus stearothermophilus | |
0.026 | - |
D-fructose 6-phosphate | pH 8.2, 30°C, G212V mutant PFK | Geobacillus stearothermophilus | |
0.12 | - |
ATP | pH 8.2, 30°C, native PFK | Geobacillus stearothermophilus | |
0.13 | - |
ATP | pH 8.2, 30°C, G212V mutant PFK | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Geobacillus stearothermophilus | ADP + D-fructose 1,6-bisphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
126 | - |
fructose 6-phosphate | pH 8.2, 30°C, G212V mutant PFK | Geobacillus stearothermophilus | |
149 | - |
fructose 6-phosphate | pH 8.2, 30°C, native PFK | Geobacillus stearothermophilus |