KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ATP | pH 6.0, 50°C | Desulfurococcus amylolyticus | |
0.28 | - |
ATP | pH 6.0, 85°C | Desulfurococcus amylolyticus | |
0.49 | - |
ADP | pH 6.0, 85°C | Desulfurococcus amylolyticus | |
0.57 | - |
D-fructose 6-phosphate | pH 6.0, 50°C | Desulfurococcus amylolyticus | |
1.17 | - |
D-fructose 6-phosphate | pH 6.0, 85°C | Desulfurococcus amylolyticus | |
16.7 | - |
D-fructose 1,6-bisphosphate | pH 6.0, 85°C | Desulfurococcus amylolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 12% of activity with Mg+ | Desulfurococcus amylolyticus | |
Mg2+ | required for activity | Desulfurococcus amylolyticus | |
Mg2+ | can be partially replaced by Ni2+, Mn2+ and Co2+ | Desulfurococcus amylolyticus | |
Mn2+ | 24% of activity with Mg2+ | Desulfurococcus amylolyticus | |
Ni2+ | 65% of activity with Mg2+ | Desulfurococcus amylolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
alpha4, 4 * 33000, SDS-PAGE | Desulfurococcus amylolyticus |
140000 | - |
gel filtration | Desulfurococcus amylolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfurococcus amylolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, ATP-agarose, Resource S | Desulfurococcus amylolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47 | - |
at 85°C | Desulfurococcus amylolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | reversible reaction in vivo, but not in vivo, very low activity with acetyl phosphate, UDP and GDP as phosphoryl donors, enzyme is not regulated allosterically | Desulfurococcus amylolyticus | ADP + D-fructose 1,6-bisphosphate | - |
r | |
CTP + D-fructose 6-phosphate | 55% of activity with ATP | Desulfurococcus amylolyticus | CDP + D-fructose 1,6-bisphosphate | - |
? | |
GTP + D-fructose 6-phosphate | - |
Desulfurococcus amylolyticus | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | 130% of activity with ATP | Desulfurococcus amylolyticus | IDP + D-fructose 1,6-bisphosphate | - |
? | |
UTP + D-fructose 6-phosphate | - |
Desulfurococcus amylolyticus | UDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 33000, SDS-PAGE | Desulfurococcus amylolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Desulfurococcus amylolyticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | very low activity at 40°C, approx. 50% of maximal activity at 70°C, activation energy: 42kJ/mol | Desulfurococcus amylolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
50% loss of activity after 10 min, addition of 5 mM ADP and 25 mM MgCl2 increases the stability by up to 10°C | Desulfurococcus amylolyticus |
100 | - |
approx. 70% loss of activity after 10 min in the absence of substrates | Desulfurococcus amylolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Desulfurococcus amylolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | 30% of maximal activity at pH 4.5, 80% at pH 6.0 | Desulfurococcus amylolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Desulfurococcus amylolyticus | chromatofocusing | - |
6 |