Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoenolpyruvate | 0.1 mM and 10 mM, allosteric inhibition, almost completely reversed by 2.5 mM ADP | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ATP | pH 7.0, 50°C and 75°C | Thermotoga maritima | |
1.4 | - |
ADP | pH 7.0, 75°C | Thermotoga maritima | |
7.6 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 75°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 30% of activity with Mg2+ | Thermotoga maritima | |
Mg2+ | required for activity | Thermotoga maritima | |
Mn2+ | - |
Thermotoga maritima | |
Zn2+ | 70% of activity with Mg2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
alpha4, 4 * 34000, SDS-PAGE | Thermotoga maritima |
140000 | - |
gel filtration | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | Thermotoga maritima | - |
ADP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose, ATP-agarose, Uno S1 | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
at 50°C | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Thermotoga maritima | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | specific for fructose 6-phosphate, very low activity with UTP and CTP | Thermotoga maritima | ADP + D-fructose 1,6-bisphosphate | - |
r | |
ATP + D-fructose 6-phosphate | reversible reaction in vitro | Thermotoga maritima | ADP + D-fructose 1,6-bisphosphate | - |
r | |
GTP + D-fructose 6-phosphate | 64% of activity of ATP | Thermotoga maritima | GDP + D-fructose 1,6-bisphosphate | - |
? | |
ITP + D-fructose 6-phosphate | 38% of activity with ATP | Thermotoga maritima | IDP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 34000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
93 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 100 | very low activity at 20°C, exponential increase in activity between 40°C and 93°C, approx. 50% of maximal activity at 80°C, activation energy: 54 kJ/mol | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
less than 20% loss of activity after 2 h | Thermotoga maritima |
80 | - |
less than 20% loss of activity after 2 h | Thermotoga maritima |
95 | - |
complete loss of activity after 1 h | Thermotoga maritima |
100 | - |
complete loss of activity after 20 min, 40% loss of activity after 2 h in the presence of 1 M ammonium sulfate | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
forward direction, at 75°C, 50% of maximal activity at pH 5.7, 80% at pH 7.7 | Thermotoga maritima |