Protein Variants | Comment | Organism |
---|---|---|
M169A | 142fold lower kcat than wild-type | Escherichia coli |
M169L | 6fold lower kcat than wild-type | Escherichia coli |
R25S | not activated by GDP, pattern of ATP inhibition nearly identical to wild-type | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | inhibition of fructose 1-phosphate phosphorylation above 0.08 mM ATP | Escherichia coli | |
additional information | no substrate inhibition with gamma-thio-ATP | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
ATP | pH 7.2, 30°C, cosubstrate fructose 1-phosphate | Escherichia coli | |
0.096 | - |
D-fructose 6-phosphate | pH 7.2, 30°C, cosubstrate gamma-thio-ATP | Escherichia coli | |
0.1 | - |
ATP | pH 7.2, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A796 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-carboxymethyl-ATP + D-fructose 6-phosphate | 90% of activity with with ATP | Escherichia coli | 1-carboxymethyl-ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + fructose 1-phosphate | 60fold lower kcat than with fructose 6-phosphate | Escherichia coli | ADP + fructose 1,6-bisphosphate | - |
? | |
gamma-thio-ATP + fructose 6-phosphate | 3000fold lower kcat than with ATP | Escherichia coli | ADP + fructose 1-thio-phosphate-6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
D-fructose 6-phosphate | pH 7.2, 30°C, cosubstrate gamma-thio-ATP | Escherichia coli | |
49 | - |
fructose 6-phosphate | pH 7.2, 30°C, cosubstrate ATP | Escherichia coli | |
88 | - |
fructose 6-phosphate | pH 7.2, 30°C, at 2mM ATP | Escherichia coli |