Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | - |
Homo sapiens | |
additional information | not activated by D-fructose 2,6-bisphosphate | Dictyostelium discoideum |
Cloned (Comment) | Organism |
---|---|
expressed in pfk-deficient Saccharomyces cerevisiae strain HD152-1D | Homo sapiens |
expressed in pfk-deficient Saccharomyces cerevisiae strain HD152-1D | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
additional information | not inhibited by ATP | Dictyostelium discoideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
ATP | wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C | Dictyostelium discoideum | |
0.07 | - |
ATP | wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Homo sapiens | |
Mg2+ | required for activity | Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
10% (w/v) PEG precipitation, DE52 column chromatography, Blue-Sepharose column chromatography, phosphocellulose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
10% (w/v) PEG precipitation, DE52 column chromatography, Blue-Sepharose column chromatography, phosphocellulose column chromatography, and Superdex 200 gel filtration | Dictyostelium discoideum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Homo sapiens | - |
muscle | - |
Dictyostelium discoideum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-fructose 6-phosphate | - |
Homo sapiens | ADP + D-fructose 1,6-bisphosphate | - |
? | |
ATP + D-fructose 6-phosphate | - |
Dictyostelium discoideum | ADP + D-fructose 1,6-bisphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-phosphofructo-1-kinase | - |
Homo sapiens |
6-phosphofructo-1-kinase | - |
Dictyostelium discoideum |
PFK | - |
Homo sapiens |
PFK | - |
Dictyostelium discoideum |
phosphofructokinase | - |
Homo sapiens |
phosphofructokinase | - |
Dictyostelium discoideum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
236 | - |
ATP | wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C | Dictyostelium discoideum | |
357 | - |
ATP | wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
ATP | wild type enzyme, at pH 7.0 in 50 mM HEPES, 100 mM KCl, 5 mM MgCl2 and 25°C | Homo sapiens |