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Literature summary for 2.7.1.114 extracted from

  • Grivell, A.R.; Jackson, J.F.
    Thymidine phosphotransferase and nucleotide phosphohydrolase of the fern Asplenium nidus. General properties and inhibition by adenosine 3:5-cyclic monophosphate (1976), Biochem. J., 155, 571-581.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.114

General Stability

General Stability Organism
bovine serum albumin stabilizes Asplenium nidus
not stabilized by AMP or thymidine Asplenium nidus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Asplenium nidus
3',5'-cAMP
-
 Asplenium nidus
adenosine
-
 Asplenium nidus
CoCl2
-
 Asplenium nidus
MnCl2
-
 Asplenium nidus
NiCl2
-
 Asplenium nidus
uridine
-
 Asplenium nidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 AMP 38°C, pH 7.5 Asplenium nidus
0.84 0.88 thymidine 38°C, pH 7.5 Asplenium nidus
1.28
-
 adenosine 38°C, pH 7.5 Asplenium nidus
4.71
-
 uridine 38°C, pH 7.5 Asplenium nidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + thymidine Hordeum vulgare
-
 adenosine + dTMP
-
 ?
AMP + thymidine Medicago sativa
-
 adenosine + dTMP
-
 ?
AMP + thymidine Helianthus tuberosus
-
 adenosine + dTMP
-
 ?
AMP + thymidine Asplenium nidus
-
 adenosine + dTMP
-
 ?
AMP + thymidine Epidendrum hybrid
-
 adenosine + dTMP
-
 ?

Organism

Organism UniProt Comment Textmining
Asplenium nidus
-
 bird's nest fern
-
Epidendrum hybrid
-
 crucifix orchid
-
Helianthus tuberosus
-
 Jerusalem artichoke
-
Hordeum vulgare
-
 barley
-
Medicago sativa
-
 lucerne
-

Purification (Commentary)

Purification (Comment) Organism
partial Asplenium nidus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Helianthus tuberosus
-
leaf
-
 Asplenium nidus
-
leaf
-
 Epidendrum hybrid
-
pollen
-
 Medicago sativa
-
root
-
 Hordeum vulgare
-
stem
-
 Helianthus tuberosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 leaf Medicago sativa
0.01
-
 stem Helianthus tuberosus
0.05
-
 leaf Epidendrum hybrid
0.05
-
 roots Hordeum vulgare
0.06
-
 tops Hordeum vulgare
0.07
-
 senescent leaf Asplenium nidus
0.29
-
 mature leaf Helianthus tuberosus
0.47
-
 young and expanded leaf Helianthus tuberosus
0.84
-
 mature leaf Asplenium nidus
0.91
-
 young mature leaf Asplenium nidus
1.09
-
 immature leaf Asplenium nidus
1.64
-
 young bud Asplenium nidus

Storage Stability

Storage Stability Organism
0°C, 6 months, 20% loss of activity Asplenium nidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + 5-bromodeoxyuridine
-
 Asplenium nidus adenosine + 5-bromodeoxyuridine 5'-phosphate
-
 ?
AMP + adenosine
-
 Asplenium nidus adenosine + AMP
-
 ?
AMP + cytidine
-
 Asplenium nidus adenosine + cytidine 5'-phosphate
-
 ?
AMP + deoxyuridine
-
 Asplenium nidus adenosine + deoxyuridine 5'-phosphate
-
 ?
AMP + guanosine
-
 Asplenium nidus adenosine + guanosine 5'-phosphate
-
 ?
AMP + thymidine
-
 Hordeum vulgare adenosine + dTMP
-
 ?
AMP + thymidine
-
 Medicago sativa adenosine + dTMP
-
 ?
AMP + thymidine
-
 Helianthus tuberosus adenosine + dTMP
-
 ?
AMP + thymidine
-
 Asplenium nidus adenosine + dTMP
-
 ?
AMP + thymidine
-
 Epidendrum hybrid adenosine + dTMP
-
 ?
AMP + thymidine AMP can be substituted by GMP, dAMP, CMP, UMP, dUMP, dTMP Asplenium nidus adenosine + dTMP
-
 ?
AMP + uridine
-
 Asplenium nidus adenosine + uridine 5'-phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 60 min, 95% loss of activity Asplenium nidus
63
-
 5 min, complete inactivation Asplenium nidus