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Literature summary for 2.7.1.12 extracted from
- Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: cloning and expression of gntP and gntK in Escherichia coli (2008), Biol. Res., 41, 349-358.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| gluconate | 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions | Corynebacterium glutamicum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli | Corynebacterium glutamicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
predicted from 167 amino acids of gluconate kinase | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + ATP | Corynebacterium glutamicum | - |
6-phospho-D-gluconate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | Q8NMT0 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Corynebacterium glutamicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.047 | - |
activity of glucokinase in Corynebacterium glutamicum (ATCC 13032) culture in MCGC medium at 37°C, supplemented with 0.5% gluconate | Corynebacterium glutamicum |
| 0.388 | - |
activity of glucokinase expressed in Escherichia strain M1-8, supplemented with fructose as carbon source | Corynebacterium glutamicum |
| 1.429 | - |
transformed Escherichia coli cells (strain DH5alpha carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source | Corynebacterium glutamicum |
| 2.894 | - |
transformed Escherichia coli cells (mutant TGN282 carrying the pTAEK plasmid), grown in Lb and collected during the exponential phase, then cultivated in CAA medium with 0.2% fructose as carbon source | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-gluconate + ATP | - |
Corynebacterium glutamicum | 6-phospho-D-gluconate + ADP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gluconokinase | - |
Corynebacterium glutamicum |
| gntK | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Corynebacterium glutamicum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | pentose phosphate pathway | Corynebacterium glutamicum |
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