Literature summary for 2.7.1.13 extracted from

Frampton, E.W.; Wood, W.A.
Purification and properties of 2-ketogluconokinase from Aerobacter aerogenes (1961), J. Biol. Chem., 236, 2578-2580.

Search for more literature for 2.7.1.13

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	stimulates	Klebsiella aerogenes
glutathione	stimulates	Klebsiella aerogenes

General Stability

General Stability	Organism
activity is lost upon dialysis	Klebsiella aerogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.6	-	2-dehydro-D-gluconate	pH 7.5, 37°C	Klebsiella aerogenes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolutely required for activity	Klebsiella aerogenes

Organism

Organism	UniProt	Comment	Textmining
Klebsiella aerogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.083	-	-	Klebsiella aerogenes

Storage Stability

Storage Stability	Organism
-14°C, 14 months, 40% loss of activity	Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2-dehydro-D-gluconate	i.e. 2-dehydro-D-gluconate	Klebsiella aerogenes	ADP + 2-dehydro-6-phosphogluconate	i.e. 6-phospho-2-dehydro-D-gluconate	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Klebsiella aerogenes

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.9	8	-	Klebsiella aerogenes

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Release 2023.1 (January 2023)