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Literature summary for 2.7.1.138 extracted from

  • Kolesnick, R.N.; Hemer, M.R.
    Characterization of a ceramide kinase activity from human leukemia (HL-60) cells. Separation from diacylglycerol kinase activity (1990), J. Biol. Chem., 265, 18803-18808.
    View publication on PubMed
Search for more literature for 2.7.1.138

General Stability

General Stability Organism
20% glycerol and 0.05 mM ATP stabilize during solubilization Homo sapiens
solubilized enzyme loses activity overnight at 4°C Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by 1,2-diacylglycerol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 ATP pH 7.0, 22°C Homo sapiens
0.045
-
 ceramide pH 7.0, 22°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome membrane Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity, 60 nM - 3 mM, 20fold linear increase in activity, progressive loss of activity at higher concentrations Homo sapiens
Mg2+ 0.3-30 mM stimulates Homo sapiens
additional information no stimulation by Mn2+, Ba2+, Cd2+ and Zn2+ Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leukemia cell line cell line HL-60 Homo sapiens
-

Storage Stability

Storage Stability Organism
-70°C, 48 h, 50% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide no activity with 1,2-diacylglycerol Homo sapiens ADP + ceramide 1-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 approx. 20% of maximal activity at pH 5.5 and pH 8.0 Homo sapiens