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Literature summary for 2.7.1.138 extracted from
- Further characterization of mammalian ceramide kinase. Substrate delivery and (stereo)specificity, tissue distribution and subcellular localization studies (2006), J. Lipid Res., 47, 268-283.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, stimulatory effect in the absence of Mg2+ | Homo sapiens |  |
| Mg2+ | 3 mM, highly dependent on Mg2+ | Homo sapiens | |
| additional information | (2R)-isomers of (2S)-substrates are stimulatory | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in CHO cells | Homo sapiens |
| expressed in Escherichia coli Top10F cells | Homo sapiens |
| expression in CHO cells in the plasma membrane | Mus musculus |
| expression in Escherichia coli strain BL21(DE3), expression in CHO cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a truncated mutant enzyme, lacking the first 115 amno acids, is not active and fails to be located in the plasma membrane | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3-cholamidopropyl-dimethylammonio-1-propanesulfonate | almost complete inhibition at high concentrations | Homo sapiens | |
| Ca2+ | above 1 mM; inhibition above 1 mM | Homo sapiens | |
| D-erythro-N,N-dimethylsphingosine | - |
Homo sapiens | |
| lauryldimethylammonium N-oxide | inhibitory at low concentrations | Homo sapiens | |
| additional information | no inhibition by ceramide analogues containing a carboxy group, i.e. N-acylated amino acids | Homo sapiens | |
| N-acetyl-D-erythro-sphingenine | substrate inhibition above 0.1 mM | Homo sapiens | |
| N-laurylsarcosine | inhibitory at low concentrations | Homo sapiens | |
| octyl-beta-D-glucopyranoside | almost complete inhibition at high concentrations | Homo sapiens | |
| sphinganine | - |
Homo sapiens | |
| sphingenine | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of recombinant enzymes | Homo sapiens | |
| 0.02 | - |
N-acetyl-D-erythro-sphingenine | pH 7.2, 37°C, recombinant enzyme | Homo sapiens | |
| 0.022 | - |
N-acetyl-sphingenine | - |
Homo sapiens | |
| 0.03 | - |
N-hexanoyl-sphingenine | - |
Homo sapiens | |
| 0.03 | - |
N-hexanoyl-D-erythro-sphingenine | pH 7.2, 37°C, recombinant enzyme | Homo sapiens | |
| 0.4 | - |
ATP | in the presence of 3 mM Mg2+ | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | bound | Mus musculus | 16020 | - |
| microsome | - |
Mus musculus | - |
- |
| microsome | in brain and testis | Rattus norvegicus | - |
- |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | in testis | Rattus norvegicus | 5739 | - |
| plasma membrane | localization via the pleckstrin homology domain at the N-terminus | Mus musculus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates to a lesser extent than Mg2+ | Homo sapiens | |
| Ca2+ | 1 mM, stimulatory effect in the absence of Mg2+ | Homo sapiens | |
| Mg2+ | at 3 mM MgCl2 | Mus musculus | |
| Mg2+ | at 3 mM MgCl2 | Rattus norvegicus | |
| Mg2+ | the recombinant enzyme is highly dependent on Mg2+, at 3 mM MgCl2, preferred divalent cation | Homo sapiens | |
| Mg2+ | 3 mM, highly dependent on Mg2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + C16-ceramide | Mus musculus | - |
ADP + C16-ceramide 1-phosphate | - |
? | |
| ATP + C16-ceramide | Homo sapiens | - |
ADP + C16-ceramide 1-phosphate | - |
? | |
| ATP + ceramide | Mus musculus | - |
ADP + ceramide 1-phosphate | - |
? | |
| ATP + ceramide | Rattus norvegicus | - |
ADP + ceramide 1-phosphate | - |
? | |
| ATP + ceramide | Homo sapiens | - |
ADP + ceramide 1-phosphate | - |
? | |
| ATP + N-hexadecanoyl-sphingenine | Homo sapiens | - |
ADP + N-hexadecanoyl-sphingenine 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q8TCT0 | - |
- |
| Mus musculus | - |
- |
- |
| Mus musculus | - |
male C57/B16 N mice | - |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | - |
male Wistar rats | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, subcellular fractionation | Mus musculus |
| partially, subcellular fractionation | Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + ceramide = ADP + ceramide 1-phosphate | the secondary hydroxy group is not required for substrate recognition, preference for the D-erythro isomer, the enzyme is specific for th phosphorylation of the 1-hydroxy group | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Mus musculus | - |
| brain | - |
Rattus norvegicus | - |
| cerebellum | - |
Mus musculus | - |
| cerebellum | high activity | Homo sapiens | - |
| cerebrum | - |
Mus musculus | - |
| cerebrum | - |
Homo sapiens | - |
| heart | high activity | Mus musculus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Mus musculus | - |
| pancreas | - |
Mus musculus | - |
| pancreas | - |
Homo sapiens | - |
| skeletal muscle | low activity | Homo sapiens | - |
| spleen | high activity | Mus musculus | - |
| testis | - |
Mus musculus | - |
| testis | - |
Rattus norvegicus | - |
| testis | high activity | Mus musculus | - |
| testis | high activity | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.92 | 5.4 | recombinant enzyme in CHO cell extract, substrate N-C6-D-erythro-sphingenine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + C16-ceramide | - |
Mus musculus | ADP + C16-ceramide 1-phosphate | - |
? | |
| ATP + C16-ceramide | - |
Homo sapiens | ADP + C16-ceramide 1-phosphate | - |
? | |
| ATP + ceramide | - |
Mus musculus | ADP + ceramide 1-phosphate | - |
? | |
| ATP + ceramide | - |
Rattus norvegicus | ADP + ceramide 1-phosphate | - |
? | |
| ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
| ATP + ceramide | in vitro substrate delivery of short chain ceramides requires bovine serum albumin, and a mixed micellar form consisting of octylglucoside and cardiolipin for long chain ceramides, choice of delivery system is important for in vitro activity | Homo sapiens | ADP + ceramide 1-phosphate | - |
? | |
| ATP + D-erythro-N-hexanoyl-sphinganine | - |
Homo sapiens | ADP + D-erythro-N-hexanoyl-sphinganine 1-phosphate | - |
? | |
| ATP + D-erythro-N-hexanoyl-sphingenine | - |
Homo sapiens | ADP + D-erythro-N-hexanoyl-sphingenine 1-phosphate | - |
? | |
| ATP + N-acetyl-D-erythro-sphingenine | - |
Homo sapiens | ADP + N-acetyl-D-erythro-sphingenine 1-phosphate | - |
? | |
| ATP + N-acetyl-sphingenine | - |
Homo sapiens | ADP + N-acetyl-sphingenine 1-phosphate | - |
? | |
| ATP + N-hexadecanoyl-sphingenine | - |
Homo sapiens | ADP + N-hexadecanoyl-sphingenine 1-phosphate | - |
? | |
| ATP + N-hexadecanoyl-sphingenine | very low activity | Homo sapiens | ADP + N-hexadecanoyl-sphingenine 1-phosphate | - |
? | |
| ATP + N-hexanoyl-1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol | - |
Homo sapiens | ADP + ? | - |
? | |
| ATP + N-hexanoyl-D-erythro-sphingenine | best substrate | Homo sapiens | ADP + N-hexanoyl-D-erythro-sphingenine 1-phosphate | - |
? | |
| ATP + N-hexanoyl-sphingenine | - |
Homo sapiens | ADP + N-hexanoyl-sphingenine 1-phosphate | - |
? | |
| ATP + N-tetradecanoyl-(2S)-amino-butan-1-ol | - |
Homo sapiens | ADP + ? | - |
? | |
| ATP + N-tetradecanoyl-(2S)-amino-hexadecan-1-ol | - |
Homo sapiens | ADP + ? | - |
? | |
| ATP + N-tetradecanoyl-2-amino-1-butanol | - |
Homo sapiens | ADP + ? | - |
? | |
| ATP + N-tetradecanoyl-2S-amino-1-butanol | - |
Homo sapiens | ADP + ? | - |
? | |
| additional information | substrate specificity, stereochemistry | Homo sapiens | ? | - |
? | |
| additional information | 2-amino-1-hexadecanol, 1-O-hexadecyl-2-deoxy-2-amino-sn-glycerol, N-tetradecanoyl-2(R)-pyrrolidine-methanol, N-tetradecanoyl-(R)-serine, N-tetradecanoyl-3-hydroxypyrrolidin, N-tetradecanoyl-4-amino-1-butanol, N-tetradecanoyl-3-amino-1-propanol, N-tetradecanoyl-(S)-serine, N-tetradecanoyl-2-benzylserine, N-tetradecanoyl3-phenylserine, N-tetradecanoyl-3-amino-tyrosine, N-tetradecanoyl-1-amino-2-naphthol-4-sulfonic acid, N-tetradecanoyl-3-hydroxy-anthranilic acid, N-tetradecanoyl-4-aminophenol, N-tetradecanoyl-diethanolamine, N-tetradecanoyl-ethanolamine, and N-tetradecanoyl-(S)-phenylalaninol are no substrates | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CERK | - |
Mus musculus |
| CERK | - |
Rattus norvegicus |
| CERK | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.8 | - |
Homo sapiens |
| 7.2 | - |
assay at | Mus musculus |
| 7.2 | - |
assay at | Rattus norvegicus |
| 7.2 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
| ATP | - |
Rattus norvegicus | |
| ATP | - |
Homo sapiens | |
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