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Literature summary for 2.7.1.138 extracted from

  • Rovina, P.; Jaritz, M.; Hoefinger, S.; Graf, C.; Devay, P.; Billich, A.; Baumruker, T.; Bornancin, F.
    A critical beta6-beta7 loop in the pleckstrin homology domain of ceramide kinase (2006), Biochem. J., 400, 255-265.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.138

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K68A/K74A/K80A shows wild type activity Homo sapiens
K90V/K98V shows partial activity Homo sapiens
K90V/R91A shows partial activity Homo sapiens
K90V/R91A/R96A/K98V totally devoid of activity Homo sapiens
R29A/R33A/R36A shows wild type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 ATP assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
0.052
-
 ATP assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
0.194
-
 ceramide assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens
0.205
-
 ceramide assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
Golgi apparatus
-
 Homo sapiens 5794
-
membrane
-
 Homo sapiens 16020
-
nucleus
-
 Homo sapiens 5634
-
vesicle
-
 Homo sapiens 31982
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8TCT0
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose-bead chromatography and Bio-Spin 6 column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
30°C, 50 mM Tris/HCl, pH 7.5, 270 mM sucrose, 1 mM EDTA, 1 mM EGTA, 5 mM tris(hydroxypropyl)phosphine, 1% Triton X-100, 20 min, 50% decrease of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ceramide
-
 Homo sapiens ADP + ceramide 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CERK
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens