Literature summary for 2.7.1.144 extracted from

Miallau, L.; Hunter, W.N.; McSweeney, S.M.; Leonard, G.A.
Structures of Staphylococcus aureus D-tagatose-6-phosphate kinase implicate domain motions in specificity and mechanism (2007), J. Biol. Chem., 282, 19948-19957.

Search for more literature for 2.7.1.144

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Staphylococcus aureus
native enzyme and L124M, L125M double mutant expressed in Escherichia coli BL21(DE3)	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
high resolution structures of D-tagatose-6-phosphate kinase (LacC) in two crystal forms. The structures define LacC in apoform, in binary complexes with ADP or the cofactor analogue adenosine 5'-(beta,gamma-imino)triphosphate, and in a ternary complex with adenosine 5'-(beta,gamma-imino)triphosphate and D-tagatose-6-phosphate. LacC adopts a closed structure required for phosphoryl transfer only when both substrate and co-factor are bound	Staphylococcus aureus
native and L124M, L125M double mutant crystallized by hanging drop vapor diffusion method in apoform, in binary complexes with ADP or the cofactor analogue AMP-PNP, and in a ternary complex with AMP-PNP and D-tagatose-6-phosphate, wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals	Staphylococcus aureus

Crystallization (Comment)

Organism

high resolution structures of D-tagatose-6-phosphate kinase (LacC) in two crystal forms. The structures define LacC in apoform, in binary complexes with ADP or the cofactor analogue adenosine 5'-(beta,gamma-imino)triphosphate, and in a ternary complex with adenosine 5'-(beta,gamma-imino)triphosphate and D-tagatose-6-phosphate. LacC adopts a closed structure required for phosphoryl transfer only when both substrate and co-factor are bound

Staphylococcus aureus

native and L124M, L125M double mutant crystallized by hanging drop vapor diffusion method in apoform, in binary complexes with ADP or the cofactor analogue AMP-PNP, and in a ternary complex with AMP-PNP and D-tagatose-6-phosphate, wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals

Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
L124M/L125M	wild-type protein give crystals that diffracte only to low resolution, the SeMet double mutant protein produced much more ordered crystals	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required, two ions bound to the active site when substrate and cofactor are bound	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-tagatose 6-phosphate	Staphylococcus aureus	second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose	ADP + D-tagatose 1,6-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	P0A0B9	-	-

Purification (Commentary)

Purification (Comment)	Organism
native and mutant protein from Escherichia coli	Staphylococcus aureus

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate	mechanism suggested	Staphylococcus aureus	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-tagatose 6-phosphate	second step of the D-tagatose-6-phosphate pathway which is the direct catabolic pathway for lactose	Staphylococcus aureus	ADP + D-tagatose 1,6-bisphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	gel filtration data, crystal structure analysis, two monomers associate via interactions between their lid domains, which come together to form a beta-barrel structure known as a beta-clasp	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
D-tagatose-6-phosphate kinase	-	Staphylococcus aureus
Lacc	-	Staphylococcus aureus
Lacc	belongs to the pfkB family of carbohydrate kinases, which form a subset of the ribokinase superfamily	Staphylococcus aureus
phosphotagatokinase	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Staphylococcus aureus