Literature summary for 2.7.1.145 extracted from

Mikkelsen, N.E.; Johansson, K.; Karlsson, A.; Knecht, W.; Andersen, G.; Piskur, J.; Munch-Petersen, B.; Eklund, H.
Structural basis for feedback inhibition of the deoxyribonucleoside salvage pathway: studies of the Drosophila deoxyribonucleoside kinase (2003), Biochemistry, 42, 5706-5712.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with dT and dTPP, hanging drop vapour diffusion method, enzyme solution containing 5-10 mg/ml protein, 5 mM dT or dTTP, is mixed in equal volumes with cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 18-22% w/v PEG 5000 monomethylether, and 5-10% w/v PEG 400, 3-5 days, 14°C, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution	Drosophila melanogaster

Crystallization (Comment)

Organism

purified recombinant enzyme in complex with dT and dTPP, hanging drop vapour diffusion method, enzyme solution containing 5-10 mg/ml protein, 5 mM dT or dTTP, is mixed in equal volumes with cyrstallization solution containing 0.1 M MES, pH 6.5, 0.2 M ammonium sulfate, 18-22% w/v PEG 5000 monomethylether, and 5-10% w/v PEG 400, 3-5 days, 14°C, X-ray diffraction structure determination and analysis at 2.4-2.5 A resolution

Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
dTTP	competitive versus ATP inpresence of 2'-deoxythymidine as substrate, feedback inhibition, mechanism, binding structure at the active site, overview	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
ATP + 2'-deoxyadenosine	Drosophila melanogaster	-	ADP + 2'-deoxyadenosine 5'-phosphate	-	?
ATP + 2'-deoxycytidine	Drosophila melanogaster	-	ADP + 2'-deoxycytidine 5'-phosphate	-	?
ATP + 2'-deoxyguanosine	Drosophila melanogaster	-	ADP + 2'-deoxyguanosine 5'-phosphate	-	?
ATP + 2'-deoxythymidine	Drosophila melanogaster	-	ADP + 2'-deoxythymidine 5'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q9XZT6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 2'-deoxyadenosine	-	Drosophila melanogaster	ADP + 2'-deoxyadenosine 5'-phosphate	-	?
ATP + 2'-deoxycytidine	-	Drosophila melanogaster	ADP + 2'-deoxycytidine 5'-phosphate	-	?
ATP + 2'-deoxyguanosine	-	Drosophila melanogaster	ADP + 2'-deoxyguanosine 5'-phosphate	-	?
ATP + 2'-deoxythymidine	-	Drosophila melanogaster	ADP + 2'-deoxythymidine 5'-phosphate	-	?
ATP + 2'-deoxythymidine	2'-deoxythymidine binding structure at the active site, overview	Drosophila melanogaster	ADP + 2'-deoxythymidine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
deoxyribonucleoside kinase	-	Drosophila melanogaster
dNK	-	Drosophila melanogaster

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	feedback inhibition kinetics	Drosophila melanogaster