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Literature summary for 2.7.1.145 extracted from

  • Knecht, W.; Mikkelsen, N.E.; Clausen, A.R.; Willer, M.; Eklund, H.; Gojkovic, Z.; Piskur, J.
    Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine (2009), Biochem. Biophys. Res. Commun., 382, 430-433.
    View publication on PubMed
Search for more literature for 2.7.1.145

Crystallization (Commentary)

Crystallization (Comment) Organism
truncated dNK lacking the last 20 amino acid residues in complex with gemcitabine, hanging drop vapor diffusion method, using 0.1 M MES pH 6.5, 0.2 M lithium sulfate and 26% (w/v) mPEG2000 Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0207
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9XZT6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxy-2',2'-difluorocytidine 2'-deoxy-2',2'-difluorocytidine is gemcitabine, dNK is an activator of gemcitabine Drosophila melanogaster ADP + difluorodeoxycytidine 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
dNK
-
 Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
314
-
 2'-deoxy-2',2'-difluorocytidine
-
 Drosophila melanogaster