Literature summary for 2.7.1.146 extracted from

Jeong, J.J.; Fushinobu, S.; Ito, S.; Shoun, H.; Wakagi, T.
Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis (2003), Acta Crystallogr. Sect. D, 59, 1327-1329.

Search for more literature for 2.7.1.146

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109	Thermococcus litoralis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25°C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution	Thermococcus litoralis

Crystallization (Comment)

Organism

purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25°C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution

Thermococcus litoralis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.56	-	D-fructose 6-phosphate	-	Thermococcus litoralis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	2 * 52000, recombinant enzyme, SDS-PAGE	Thermococcus litoralis
125000	-	recombinant enzyme, gel filtration	Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ADP + D-fructose 6-phosphate	Thermococcus litoralis	-	AMP + D-fructose 1,6-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus litoralis	Q977Q3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration	Thermococcus litoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
157	-	purified recombinant enzyme	Thermococcus litoralis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ADP + D-fructose 6-phosphate	-	Thermococcus litoralis	AMP + D-fructose 1,6-bisphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 52000, recombinant enzyme, SDS-PAGE	Thermococcus litoralis

Synonyms

Synonyms	Comment	Organism
ADP-dependent phosphofructokinase	-	Thermococcus litoralis
TLPFK	-	Thermococcus litoralis

Cofactor

Cofactor	Comment	Organism	Structure
ADP	-	Thermococcus litoralis
additional information	no activity with ATP	Thermococcus litoralis