Cloned (Comment) | Organism |
---|---|
gene PH0589, DNA and amino acid sequence determination and analysis, expression of the N-terminally His-tagged in Escherichia coli | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
purified recombinant N-terminally His-tagged apoenzyme, hanging drop vapour diffusion method, 20°C, 10 mg/ml protein in solution is mixed with an equal volume of 0.003 ml of mother liquor containing 9-13% PEG 6000, 0.2 M Li2SO4, and 0.1 M citrate buffer, pH 3.6, heavy atom derivatization with HgCl2, X-ray diffraction structure determination and analysis at 2.0 A resolution, single isomorphous replacement with an anomalous scattering | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucose | Pyrococcus horikoshii | - |
AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | Pyrococcus horikoshii OT-3 | - |
AMP + D-glucose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
strain OT3, gene PH0589 | - |
Pyrococcus horikoshii OT-3 | - |
strain OT3, gene PH0589 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli by heat treatment and nickel affinity chromatography | Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity determination in a coupled assay with glucose 6-phosphate dehydrogenase and NADP+ | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucose | - |
Pyrococcus horikoshii | AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | - |
Pyrococcus horikoshii OT-3 | AMP + D-glucose 6-phosphate | - |
? | |
additional information | substrate-induced fit, binding of ADP induces large conformational changes | Pyrococcus horikoshii | ? | - |
? | |
additional information | substrate-induced fit, binding of ADP induces large conformational changes | Pyrococcus horikoshii OT-3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-dependent glucokinase | - |
Pyrococcus horikoshii |
ADPGK | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | binding site structure composed of 2 loops | Pyrococcus horikoshii |