Cloned (Comment) | Organism |
---|---|
gene ispE, expression of the His-tagged in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in ternary complex with substrate and non-hydrolyzable ATP analogue adenosine 5'-[beta,gamma-imino]triphosphate, i.e. AMP-PNP, 25 mg/ml protein in 50 mM Tris-HCl, pH 7.7, 50 mM NaCl, 3 mM AMP-PNP, and 2 mM substrate 4-(cytidine 5'-phosphate)-2-C-methyl-D-erythritol, hanging drop vapour diffusion method, 0.001 ml protein solution mixed with equal volume of reservoir solution containing 20% polyethylene glycol 8000, 0.2 M magnesium acetate, 0.1 M sodium cacodylate, pH 6.5, and 0.0002 ml of 0.25 sulfo-betaine, cryoprotection at -173°C by 20% glycerol, X-ray diffraction structure determination and analysis at 2.0 A resolution | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Escherichia coli | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene ispE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain BL21(DE3) to homogeneity by nickel affinity and anion exchange chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | reaction mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis | Escherichia coli | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | i.e. CDP-ME | Escherichia coli | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme subunit displays the alpha/beta fold characteristic of the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily, arranged into cofactor and substrate-binding domains with the catalytic center positioned in a deep cleft between domains, quarternary structure, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
4-diphosphocytidyl-2C-methyl-D-erythritol kinase | - |
Escherichia coli |
More | the enzyme belongs to the galactose kinase/homoserine kinase/mevalonate kinase phosphomevalonate kinase superfamily | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |