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Literature summary for 2.7.1.148 extracted from

  • Gimenez-Oya, V.; Villacanas, O.; Fernandez-Busquets, X.; Rubio-Martinez, J.; Imperial, S.
    Mimicking direct protein-protein and solvent-mediated interactions in the CDP-methylerythritol kinase homodimer: a pharmacophore-directed virtual screening approach (2009), J. Mol. Model., 15, 997-1007.
    View publication on PubMed
Search for more literature for 2.7.1.148

Application

Application Comment Organism
drug development enzymes of the MEP pathway represent potential targets for the generation of selective antibacterial, antimalarial and herbicidal molecules Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure is resolved by X-ray diffraction at a resolution of 2.01 A Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 determined by MALDI-TOF-MS Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Escherichia coli
-
 ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-trap chelating columns are used for the purification of recombinant 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 Escherichia coli ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

Subunits Comment Organism
homodimer determined by gel filtration, asymmetric unit consists of 2 subunits A and B which assemble with C2 symmetry to form an extended homodimer, but only 4% of the total surface area of the 2 monomers is involved in dimer formation Escherichia coli

Synonyms

Synonyms Comment Organism
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase
-
 Escherichia coli
CDP-methylerythritol kinase
-
 Escherichia coli
CMK
-
 Escherichia coli

General Information

General Information Comment Organism
metabolism enzyme catalyses the fourth reaction step of the 2C-methylerythritol 4-phosphate (MEP) pathway for the biosynthesis of isopentenyl pyrophosphate and its isomer dimethylallyl pyrophosphate Escherichia coli
physiological function the MEP pathway is essential in the malaria parasite Plasmodium falciparum and in most eubacteria, including the causal agents for diverse and serious human diseases like leprosy, bacterial meningitis, various gastrointestinal and sexually transmitted infections, tuberculosis, and certain types of pneumonia Escherichia coli