Literature summary for 2.7.1.149 extracted from

Davis, M.I.; Sasaki, A.T.; Shen, M.; Emerling, B.M.; Thorne, N.; Michael, S.; Pragani, R.; Boxer, M.; Sumita, K.; Takeuchi, K.; Auld, D.S.; Li, Z.; Cantley, L.C.; Simeonov, A.
A homogeneous, high-throughput assay for phosphatidylinositol 5-phosphate 4-kinase with a novel, rapid substrate preparation (2013), PLoS ONE, 8, e54127.

Search for more literature for 2.7.1.149

Inhibitors

Inhibitors	Comment	Organism	Structure
(2E)-2-(3,4-dihydroxybenzoyl)-3-(3,4-dihydroxyphenyl)prop-2-enenitrile	-	Homo sapiens
(2E)-2-(3,4-dihydroxybenzoyl)-3-(4-hydroxy-3-iodo-5-methoxyphenyl)prop-2-enenitrile	-	Homo sapiens
(3E)-5-amino-3-[(2Z)-1-cyano-2-(3H-indol-3-ylidene)ethylidene]-2,3-dihydro-1H-pyrazole-4-carbonitrile	-	Homo sapiens
(3Z)-2-amino-4-(3,4,5-trihydroxyphenyl)buta-1,3-diene-1,1,3-tricarbonitrile	-	Homo sapiens
additional information	high-throughput inhibitor screening method, counterscreen assay and ATP competition assay, overview	Homo sapiens
tyrphostin	ATP-competitive inhibitors, i.e. tyrosine phosphorylation inhibitors, of isozyme PI5P4Kalpha	Homo sapiens
tyrphostin 23	-	Homo sapiens
tyrphostin 47	-	Homo sapiens
tyrphostin AG 1478	-	Homo sapiens
tyrphostin AG 490	-	Homo sapiens
tyrphostin AG 494	-	Homo sapiens
tyrphostin AG 537	-	Homo sapiens
tyrphostin AG 555	-	Homo sapiens
tyrphostin AG 698	-	Homo sapiens
tyrphostin AG 808	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate	Homo sapiens	-	ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate	-	Homo sapiens	ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate	-	?

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol 5-phosphate 4-kinase	-	Homo sapiens
PI5P 4-kinase	-	Homo sapiens
PI5P4K	-	Homo sapiens
PI5P4Kalpha	-	Homo sapiens
type II kinase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.001	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin
0.002	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	(2E)-2-(3,4-dihydroxybenzoyl)-3-(4-hydroxy-3-iodo-5-methoxyphenyl)prop-2-enenitrile
0.005	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	(3Z)-2-amino-4-(3,4,5-trihydroxyphenyl)buta-1,3-diene-1,1,3-tricarbonitrile
0.013	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	(3E)-5-amino-3-[(2Z)-1-cyano-2-(3H-indol-3-ylidene)ethylidene]-2,3-dihydro-1H-pyrazole-4-carbonitrile
0.014	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	(2E)-2-(3,4-dihydroxybenzoyl)-3-(3,4-dihydroxyphenyl)prop-2-enenitrile
0.018	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 808
0.02	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin 47
0.032	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 537
0.045	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin 23
0.05	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 698
0.055	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 555
0.089	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 494
0.089	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 490
0.1	-	pH 7.4, 22°C, isozyme PI5P4Kalpha	Homo sapiens	tyrphostin AG 1478

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Release 2023.1 (January 2023)